PUBLICATION
Red Fluorescent Protein Variant with a Dual-Peak Emission of Fluorescence
- Authors
- Ossa-Hernández, N., Marins, L.F., Almeida, R.V., Almeida, D.V.
- ID
- ZDB-PUB-231022-51
- Date
- 2023
- Source
- Marine biotechnology (New York, N.Y.) 25(6): 1099-1109 (Journal)
- Registered Authors
- Almeida, Daniela Volcan, Marins, Luis Fernando
- Keywords
- Chromophore, Fluorescent reporter, Random mutagenesis, Transgenic fish
- MeSH Terms
-
- Animals
- Anthozoa*/genetics
- Anthozoa*/metabolism
- Biotechnology
- Fluorescence
- Green Fluorescent Proteins
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Red Fluorescent Protein*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 37864761 Full text @ Mar. Biotechnol.
Citation
Ossa-Hernández, N., Marins, L.F., Almeida, R.V., Almeida, D.V. (2023) Red Fluorescent Protein Variant with a Dual-Peak Emission of Fluorescence. Marine biotechnology (New York, N.Y.). 25(6):1099-1109.
Abstract
The marine environment is a rich reservoir of diverse biological entities, many of which possess unique properties that are of immense value to biotechnological applications. One such example is the red fluorescent protein derived from the coral Discosoma sp. This protein, encoded by the DsRed gene, has been the subject of extensive research due to its potential applications in various fields. In the study, a variant of the red fluorescent protein was generated through random mutagenesis using the DsRed2 gene as a template. The process employed error-prone PCR (epPCR) to introduce random mutations, leading to the isolation of twelve gene variants. Among these, one variant stood out due to its unique spectral properties, exhibiting dual fluorescence emission at both 480 nm (green) and 550 nm (red). This novel variant was expressed in both Escherichia coli and zebrafish (Danio rerio) muscle, confirming the dual fluorescence emission in both model systems. One of the immediate applications of this novel protein variant is in ornamental aquaculture. The dual fluorescence can serve as a unique marker or trait, enhancing the aesthetic appeal of aquatic species in ornamental settings.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping