PUBLICATION
The effect of stabilized culture of Lactobacillus rhamnosus GCC-3 on gut and liver health, and anti-viral immunity of zebrafish
- Authors
- Liang, H., Xie, Y., Li, M., Chen, J., Zhou, W., Xia, R., Ding, Q., Yao, Y., Zhang, Z., Yang, Y., Ran, C., Zhou, Z.
- ID
- ZDB-PUB-230916-62
- Date
- 2023
- Source
- Fish & shellfish immunology 141: 109074 (Journal)
- Registered Authors
- Ding, Qianwen, Ran, Chao, Xie, Yadong, Yang, Yalin, Zhang, Zhen, Zhou, Zhigang
- Keywords
- Gut microbiota, Spring viremia of carp virus, Stabilized cultures of Lactobacillus, Zebrafish
- MeSH Terms
-
- Animal Feed/analysis
- Animals
- Antiviral Agents
- Diet/veterinary
- Lacticaseibacillus rhamnosus*
- Liver
- RNA, Ribosomal, 16S
- Zebrafish*
- PubMed
- 37714442 Full text @ Fish Shellfish Immunol.
Citation
Liang, H., Xie, Y., Li, M., Chen, J., Zhou, W., Xia, R., Ding, Q., Yao, Y., Zhang, Z., Yang, Y., Ran, C., Zhou, Z. (2023) The effect of stabilized culture of Lactobacillus rhamnosus GCC-3 on gut and liver health, and anti-viral immunity of zebrafish. Fish & shellfish immunology. 141:109074.
Abstract
Probiotics are promising antibiotics alternatives to improve growth and disease resistance of cultured fish. Our study aimed to investigate the effect of dietary stabilized culture of Lactobacillus rhamnosus GCC-3 on growth performance, gut and liver health and anti-viral ability of zebrafish (Danio rerio). Zebrafish (0.161 ± 0.001 g) were fed control and the experimental diet containing 1% GCC-3 culture (1 × 107 CFU/g diet) for four weeks. Growth performance and gut and liver health parameters were monitored after four weeks feeding. The gut microbiota was analyzed by 16S rRNA gene sequencing. In another experiment, zebrafish (0.212 ± 0.001 g) were fed with basal or GCC-3 diets and challenged by spring viremia of carp virus (SVCV) at the end of feeding. The antiviral immune response was evaluated at 2nd and 4th days post SVCV infection and survival rate was calculated 14 days after challenge. The results showed that adding 1% GCC-3 significantly improved growth performance of zebrafish (P < 0.05). The intestinal expression of hypoxia-inducible factor Hif-1α, tight junction protein ZO-1α and ZO-1β was significantly up-regulated in 1% GCC-3 group compared with control (P < 0.05). Besides, 1% GCC-3 decreased the content of MDA and increased total antioxidant capacity in the intestine, and the relative expression of SOD, GST and Gpxa was improved. The abundance of Proteobacteria was reduced while Firmicutes was enriched in the intestinal microbiota of 1% GCC-3 group compared with control (P < 0.05). Zebrafish fed 1% GCC-3 showed higher survival rate after SVCV challenge. Accordingly, the expression of antiviral genes in the spleen was increased at 2nd and 4th days post infection. In conclusion, our results indicate that dietary 1% GCC-3 supplementation can improve gut and liver health as well as antiviral immunity of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping