PUBLICATION
Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo
- Authors
- Cacialli, P., Dogan, S., Linnerz, T., Pasche, C., Bertrand, J.Y.
- ID
- ZDB-PUB-230713-37
- Date
- 2023
- Source
- Stem Cell Reports 18: 153415461534-1546 (Journal)
- Registered Authors
- Bertrand, Julien, Cacialli, Pietro, Linnerz, Tanja, Pasche, Corentin
- Keywords
- apoptosis, cell cycle, hematopoietic stem cells, hemogenic endothelium, mcm10, p53, zebrafish
- MeSH Terms
-
- Animals
- Apoptosis/genetics
- Cell Cycle Proteins
- Female
- Hemangioblasts*
- Hematopoietic Stem Cells
- Minichromosome Maintenance Proteins*
- Zebrafish*
- Zebrafish Proteins*
- PubMed
- 37437546 Full text @ Stem Cell Reports
Citation
Cacialli, P., Dogan, S., Linnerz, T., Pasche, C., Bertrand, J.Y. (2023) Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo. Stem Cell Reports. 18:153415461534-1546.
Abstract
Hematopoietic stem cells (HSCs) guarantee the continuous supply of all blood lineages during life. In response to stress, HSCs are capable of extensive proliferative expansion, whereas in steady state, HSCs largely remain in a quiescent state to prevent their exhaustion. DNA replication is a very complex process, where many factors need to exert their functions in a perfectly concerted manner. Mini-chromosome-maintenance protein 10 (Mcm10) is an important replication factor, required for proper assembly of the eukaryotic replication fork. In this report, we use zebrafish to study the role of mcm10 during embryonic development, and we show that mcm10 specifically regulates HSC emergence from the hemogenic endothelium. We demonstrate that mcm10-deficient embryos present an accumulation of DNA damages in nascent HSCs, inducing their apoptosis. This phenotype can be rescued by knocking down p53. Taken all together, our results show that mcm10 plays an important role in the emergence of definitive hematopoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping