PUBLICATION
Dynamic and broad expression of adamts9 in developing and adult zebrafish
- Authors
- He, Y., Carver, J.J., Erickson, T., Le Pabic, P., Zhu, Y.
- ID
- ZDB-PUB-230713-35
- Date
- 2023
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 252(12): 1449-1461 (Journal)
- Registered Authors
- Erickson, Timothy, Zhu, Yong
- Keywords
- brain, ciliary marginal zone, folliculogenesis, metalloprotease, retina
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Female
- Ovarian Follicle*
- Ovary
- Transgenes
- Zebrafish*
- PubMed
- 37436116 Full text @ Dev. Dyn.
Citation
He, Y., Carver, J.J., Erickson, T., Le Pabic, P., Zhu, Y. (2023) Dynamic and broad expression of adamts9 in developing and adult zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 252(12):1449-1461.
Abstract
Background Previous studies showed that Adamts9 is involved in multiple functions including ovulation, spine formation, primordial germ cell migration, and development of primary ovarian follicles in animals. However, systemic examination and high-resolution analyses of adamts9 expression are missing due to lack of a sensitive reporter assay.
Results In the present study, we created a new transgenic zebrafish reporter line Tg(adamts9:EGFP) and assayed its expression in various tissues and cells during development and in adults at high-resolution using confocal imaging. Reporter expression was validated with real-time quantitative PCR, whole mount in situ hybridization, and immunohistochemistry for endogenous adamts9. Strong expression of the adamts9:EGFP transgene was found in a wide range of adult and embryonic zebrafish tissues/cells including ovaries, testes, brains, eyes, pectoral fins, intestine, skin, gill, muscle, and heart; while lower expression was observed in the liver and growing ovarian follicles (stages II and III).
Conclusions Our results of a broad and dynamic expression pattern for this evolutionary conserved metalloprotease suggest involvement of adamts9 in the development and physiological functions of various tissues in animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping