PUBLICATION
The Opto-inflammasome in zebrafish as a tool to study cell and tissue responses to speck formation and cell death
- Authors
- Hasel de Carvalho, E., Dharmadhikari, S.S., Shkarina, K., Xiong, J.R., Reversade, B., Broz, P., Leptin, M.
- ID
- ZDB-PUB-230708-33
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Leptin, Maria, REVERSADE, Bruno
- Keywords
- 0ptogenetics, ASC speck, cell death, epithelial cells, immunology, inflammasome, inflammation, zebrafish
- MeSH Terms
-
- Animals
- Apoptosis
- CARD Signaling Adaptor Proteins/genetics
- CARD Signaling Adaptor Proteins/metabolism
- Caspase 1/metabolism
- Inflammasomes*/metabolism
- NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
- Pyroptosis
- Zebrafish*/metabolism
- PubMed
- 37417734 Full text @ Elife
Citation
Hasel de Carvalho, E., Dharmadhikari, S.S., Shkarina, K., Xiong, J.R., Reversade, B., Broz, P., Leptin, M. (2023) The Opto-inflammasome in zebrafish as a tool to study cell and tissue responses to speck formation and cell death. eLIFE. 12:.
Abstract
The inflammasome is a conserved structure for the intracellular detection of danger or pathogen signals. As a large intracellular multiprotein signaling platform, it activates downstream effectors that initiate a rapid necrotic programmed cell death (PCD) termed pyroptosis and activation and secretion of pro-inflammatory cytokines to warn and activate surrounding cells. However, inflammasome activation is difficult to control experimentally on a single-cell level using canonical triggers. We constructed Opto-ASC, a light-responsive form of the inflammasome adaptor protein ASC (Apoptosis-Associated Speck-Like Protein Containing a CARD) which allows tight control of inflammasome formation in vivo. We introduced a cassette of this construct under the control of a heat shock element into zebrafish in which we can now induce ASC inflammasome (speck) formation in individual cells of the skin. We find that cell death resulting from ASC speck formation is morphologically distinct from apoptosis in periderm cells but not in basal cells. ASC-induced PCD can lead to apical or basal extrusion from the periderm. The apical extrusion in periderm cells depends on Caspb and triggers a strong Ca2+ signaling response in nearby cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping