PUBLICATION
Acquisition of triacylglycerol transfer activity by microsomal triglyceride transfer protein during evolution
- Authors
- Rava, P., Hussain, M.M.
- ID
- ZDB-PUB-230620-77
- Date
- 2007
- Source
- Biochemistry 46: 122631227412263-74 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Biological Transport
- COS Cells
- Carrier Proteins/chemistry
- Carrier Proteins/genetics
- Carrier Proteins/metabolism*
- Chlorocebus aethiops
- Chromatography, Affinity
- DNA Primers
- Evolution, Molecular*
- Fluorescent Antibody Technique
- Humans
- Molecular Sequence Data
- Sequence Homology, Amino Acid
- Triglycerides/metabolism*
- PubMed
- 17924655 Full text @ Biochemistry
Citation
Rava, P., Hussain, M.M. (2007) Acquisition of triacylglycerol transfer activity by microsomal triglyceride transfer protein during evolution. Biochemistry. 46:122631227412263-74.
Abstract
Microsomal triglyceride transfer protein (MTP) is essential for the assembly of neutral-lipid-rich apolipoprotein B (apoB) lipoproteins. Previously we reported that the Drosophila MTP transfers phospholipids but does not transfer triglycerides. In contrast, human MTP transfers both lipids. To explore the acquisition of triglyceride transfer activity by MTP, we evaluated amino acid sequences, protein structures, and the biochemical and cellular properties of various MTP orthologues obtained from species that diverged during evolution. All MTP orthologues shared similar secondary and tertiary structures, associated with protein disulfide isomerase, localized to the endoplasmic reticulum, and supported apoB secretion. While vertebrate MTPs transferred triglyceride, invertebrate MTPs lacked this activity. Thus, triglyceride transfer activity was acquired during the transition from invertebrates to vertebrates. Within vertebrates, fish, amphibians, and birds displayed 27%, 40%, and 100% triglyceride transfer activity compared to mammals. We conclude that MTP triglyceride transfer activity first appeared in fish and speculate that the acquisition of triglyceride transfer activity by MTP provided for a significant advantage in the evolution of larger and more complex organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping