PUBLICATION
Effects of PFOS, F53-B and F53-B on locomotor behaviour, the dopamine system and mitochondrial function in developing zebrafish (Danio rerio)
- Authors
- Kalyn, M., Lee, H., Curry, J., Tu, W., Ekker, M., Mennigen, J.A.
- ID
- ZDB-PUB-230324-50
- Date
- 2023
- Source
- Environmental pollution (Barking, Essex : 1987) 326: 121479 (Journal)
- Registered Authors
- Ekker, Marc
- Keywords
- Behaviour, Dopaminergic systems, Mitochondrial homeostasis, PFOS alternatives, Zebrafish
- MeSH Terms
-
- Alkanesulfonic Acids*/metabolism
- Alkanesulfonic Acids*/toxicity
- Animals
- Animals, Wild
- Dopamine/metabolism
- Fluorocarbons*/metabolism
- Fluorocarbons*/toxicity
- Humans
- Protein Kinases/metabolism
- Water Pollutants, Chemical*/analysis
- Zebrafish/metabolism
- PubMed
- 36958660 Full text @ Environ. Pollut.
Citation
Kalyn, M., Lee, H., Curry, J., Tu, W., Ekker, M., Mennigen, J.A. (2023) Effects of PFOS, F53-B and F53-B on locomotor behaviour, the dopamine system and mitochondrial function in developing zebrafish (Danio rerio). Environmental pollution (Barking, Essex : 1987). 326:121479.
Abstract
Perfluorooctanesulfonic acid (PFOS) has widely been reported to persist in the environment and to elicit neurotoxicological effects in wildlife and humans. Following the restriction of PFOS use, 6:2 chlorinated polyfluorinated ether sulfonate (F53-B) and sodium p-perfluorous nonenoxybenzene sulfonate (OBS) have emerged as novel PFOS alternatives and have been widely detected in the environment. However, knowledge on toxicological effects of these alternatives remains scarce. Using developing transgenic Tg (dat:eGFP) zebrafish, we here evaluated the consequences of exposure to 0, 0.1 and 1 mg/l PFOS, F53-B and OBS on the dopaminergic system, locomotor behaviour and mitochondrial function. All compounds generally reduced locomotor activity under light conditions irrespective of exposure concentration. Exposure to OBS (at all concentrations), as well as PFOS and F53-B (at 1 mg/l), significantly reduced subpallial dopamine neuron abundance. PFOS also significantly reduced dat and pink1 expression irrespective of exposure concentration, while F53-B and OBS tended to reduce mitochondrial pink1 and fis1 expressions across concentrations without reaching significance. Mitochondrial function, in form of reduced oxygen consumption and marginally inhibited ATP-linked oxygen consumption rates, was affected only in response to 1 mg/l PFOS. Together, PFOS and emerging contaminants F53-B and OBS inhibit locomotion at similar concentrations, a finding correlated with decreased dopaminergic neuron numbers in the subpallium and decreased expression of pink1. These findings are relevant to wildlife and human health, as they suggest that PFOS as well as replacement compounds affect locomotion likely in part by negatively impacting the dopamine system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping