PUBLICATION
Isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate plays an anti-hypoxic role through regulating neuroactive ligand-receptor interaction signaling pathway in larval zebrafish
- Authors
- Zhang, S., Wang, X., Yang, Q., Xia, Q., Zhao, Y., Zheng, X., Zhang, Y., Liu, K.
- ID
- ZDB-PUB-230323-42
- Date
- 2023
- Source
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 161: 114570114570 (Journal)
- Registered Authors
- Keywords
- Hypoxia, IDHP, Neural active ligand-receptor interaction pathway, Transcriptome, Zebrafish larvae
- MeSH Terms
-
- Animals
- Hypoxia*/drug therapy
- Larva/metabolism
- Ligands
- Signal Transduction
- Zebrafish*
- PubMed
- 36948132 Full text @ Biomed. Pharmacother.
Citation
Zhang, S., Wang, X., Yang, Q., Xia, Q., Zhao, Y., Zheng, X., Zhang, Y., Liu, K. (2023) Isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate plays an anti-hypoxic role through regulating neuroactive ligand-receptor interaction signaling pathway in larval zebrafish. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 161:114570114570.
Abstract
Isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate (IDHP) is the core active substance of salvia miltiorrhiza in disease treatment. The significance of our work lies in evaluating the ameliorating effects of IDHP on hypoxia-induced injury and investigating its mechanisms. We examined the morphology, dopamine neurons (DANs), cerebral vessels, and behavior of zebrafish larvae administrated by IDHP/VHC after hypoxia-induction. We next sought to explore its anti-hypoxic mechanisms via transcriptome analysis and qPCR experiments. The results indicated that hypoxia-induced injuries, including decreased length of DANs, number of cereal vessels, total swimming distance, and average swimming speed, were all alleviated by IDHP. Furthermore, transcriptome analysis provided a sign that IDHP most likely played the anti-hypoxic role through the neuroactive ligand-receptor interaction (NLRI) signaling pathway. Consistently, expression of related genes, such as f2rl1.1, p2ry10, npy1r, ptger2b, ptger2b, pth2rb, and nmur1a, was downregulated by hypoxia induction and recovered after IDHP administration. Therefore, we speculated that, via regulating NLRI, IDHP reduced inflammation, promoted angiogenesis, modulated blood pressure and flow, and inhibited cell apoptosis, and eventually played an anti-hypoxic role.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping