PUBLICATION
Pathogenic variations in MAML2 and MAMLD1 contribute to congenital hypothyroidism due to dyshormonogenesis by regulating the Notch signalling pathway
- Authors
- Wu, F.Y., Yang, R.M., Zhang, H.Y., Zhan, M., Tu, P.H., Fang, Y., Zhang, C.X., Song, S.Y., Dong, M., Cui, R.J., Liu, X.Y., Yang, L., Yan, C.Y., Sun, F., Zhang, R.J., Wang, Z., Liang, J., Song, H.D., Cheng, F., Zhao, S.X.
- ID
- ZDB-PUB-230312-36
- Date
- 2023
- Source
- Journal of Medical Genetics 60(9): 874-884 (Journal)
- Registered Authors
- Dong, Mei
- Keywords
- congenital, hereditary, and neonatal diseases and abnormalities, endocrine system diseases, genetics, medical
- MeSH Terms
-
- Animals
- Congenital Hypothyroidism*/genetics
- DNA-Binding Proteins/genetics
- HEK293 Cells
- Humans
- Mice
- Mutation
- Nuclear Proteins/genetics
- Thyroid Hormones/genetics
- Trans-Activators/genetics
- Transcription Factors/genetics
- Zebrafish
- PubMed
- 36898841 Full text @ J. Med. Genet.
Citation
Wu, F.Y., Yang, R.M., Zhang, H.Y., Zhan, M., Tu, P.H., Fang, Y., Zhang, C.X., Song, S.Y., Dong, M., Cui, R.J., Liu, X.Y., Yang, L., Yan, C.Y., Sun, F., Zhang, R.J., Wang, Z., Liang, J., Song, H.D., Cheng, F., Zhao, S.X. (2023) Pathogenic variations in MAML2 and MAMLD1 contribute to congenital hypothyroidism due to dyshormonogenesis by regulating the Notch signalling pathway. Journal of Medical Genetics. 60(9):874-884.
Abstract
Background In several countries, thyroid dyshormonogenesis is more common than thyroid dysgenesis in patients with congenital hypothyroidism (CH). However, known pathogenic genes are limited to those directly involved in hormone biosynthesis. The aetiology and pathogenesis of thyroid dyshormonogenesis remain unknown in many patients.
Methods To identify additional candidate pathogenetic genes, we performed next-generation sequencing in 538 patients with CH and then confirmed the functions of the identified genes in vitro using HEK293T and Nthy-ori 3.1 cells, and in vivo using zebrafish and mouse model organisms.
Results We identified one pathogenic MAML2 variant and two pathogenic MAMLD1 variants that downregulated canonical Notch signalling in three patients with CH. Zebrafish and mice treated with N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butylester, a γ-secretase inhibitor exhibited clinical manifestations of hypothyroidism and thyroid dyshormonogenesis. Through organoid culture of primary mouse thyroid cells and transcriptome sequencing, we demonstrated that Notch signalling within thyroid cells directly affects thyroid hormone biosynthesis rather than follicular formation. Additionally, these three variants blocked the expression of genes associated with thyroid hormone biosynthesis, which was restored by HES1 expression. The MAML2 variant exerted a dominant-negative effect on both the canonical pathway and thyroid hormone biosynthesis. MAMLD1 also regulated hormone biosynthesis through the expression of HES3, the target gene of the non-canonical pathway.
Conclusions This study identified three mastermind-like family gene variants in CH and revealed that both canonical and non-canonical Notch signalling affected thyroid hormone biosynthesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping