PUBLICATION
Overexpression of miR-124-3p affects zebrafish inner ear development and hearing function via downregulation of EYA1 gene expression
- Authors
- Zhang, R., Sun, Y., Zhang, Q., Lin, J., Zhang, Y., Chen, X., Liu, T., Li, Q.
- ID
- ZDB-PUB-230312-34
- Date
- 2023
- Source
- Neuroscience letters 802: 137172 (Journal)
- Registered Authors
- Li, Qiang
- Keywords
- EYA1, Hearing, Inner ear development, MiR-124-3p, Zebrafish
- MeSH Terms
-
- 3' Untranslated Regions
- Animals
- Down-Regulation
- Ear, Inner*/metabolism
- Gene Expression
- Hearing
- MicroRNAs*/genetics
- MicroRNAs*/metabolism
- Zebrafish/metabolism
- PubMed
- 36898654 Full text @ Neurosci. Lett.
Citation
Zhang, R., Sun, Y., Zhang, Q., Lin, J., Zhang, Y., Chen, X., Liu, T., Li, Q. (2023) Overexpression of miR-124-3p affects zebrafish inner ear development and hearing function via downregulation of EYA1 gene expression. Neuroscience letters. 802:137172.
Abstract
The EYA1 gene is essential for normal inner ear development and affects the development and function of the inner ear in a dose-dependent manner. However, the mechanisms regulating EYA1 gene expression are not well understood. Recently, miRNAs have become recognized as important regulators of gene expression. In this study, we identified miR-124-3p through a microRNA (miRNA) target prediction website and found that miR-124-3p and its target site in the EYA1 3' untranslated region (3'UTR) are conserved in most vertebrates. Both in vivo and in vitro, the interaction of miR-124-3p with the EYA1 3'UTR exerts a negative regulatory effect. Microinjection of agomiR-124-3p into zebrafish embryos resulted in a phenotype of reduced auricular area, suggesting inner ear dysplasia. In addition, injection of agomiR-124-3p or antagomir caused abnormal hearing function in zebrafish. In conclusion, our results suggest that miR-124-3p can affect inner ear development and hearing function in zebrafish by regulating EYA1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping