PUBLICATION
In Vivo Fast Nonlinear Microscopy Reveals Impairment of Fast Axonal Transport Induced by Molecular Motor Imbalances in the Brain of Zebrafish Larvae
- Authors
- Grimaud, B., Frétaud, M., Terras, F., Bénassy, A., Duroure, K., Bercier, V., Trippé-Allard, G., Mohammedi, R., Gacoin, T., Del Bene, F., Marquier, F., Langevin, C., Treussart, F.
- ID
- ZDB-PUB-221203-6
- Date
- 2022
- Source
- ACS nano 16(12): 20470-20487 (Journal)
- Registered Authors
- Bercier, Valérie, Del Bene, Filippo, Duroure, Karine, Langevin, Christelle
- Keywords
- axonal transport, disease models, molecular motor, nonlinear nanocrystal, second-harmonic generation, single-nanoparticle tracking, two-photon microscopy, zebrafish larva
- MeSH Terms
-
- Animals
- Axonal Transport/genetics
- Axons
- Brain/metabolism
- Dyneins*/metabolism
- Kinesins*/metabolism
- Larva/metabolism
- Microscopy
- Microtubules/metabolism
- Zebrafish/metabolism
- PubMed
- 36459488 Full text @ ACS Nano
Citation
Grimaud, B., Frétaud, M., Terras, F., Bénassy, A., Duroure, K., Bercier, V., Trippé-Allard, G., Mohammedi, R., Gacoin, T., Del Bene, F., Marquier, F., Langevin, C., Treussart, F. (2022) In Vivo Fast Nonlinear Microscopy Reveals Impairment of Fast Axonal Transport Induced by Molecular Motor Imbalances in the Brain of Zebrafish Larvae. ACS nano. 16(12):20470-20487.
Abstract
Cargo transport by molecular motors along microtubules is essential for the function of eukaryotic cells, in particular neurons in which axonal transport defects constitute the early pathological features of neurodegenerative diseases. Mainly studied in motor and sensory neurons, axonal transport is still difficult to characterize in neurons of the brain in absence of appropriate in vivo tools. Here, we measured fast axonal transport by tracing the second harmonic generation (SHG) signal of potassium titanyl phosphate (KTP) nanocrystals (nanoKTP) endocytosed by brain neurons of zebrafish (Zf) larvae. Thanks to the optical translucency of Zf larvae and to the perfect photostability of nanoKTP SHG, we achieved a high scanning speed of 20 frames (of ≈90 μm × 60 μm size) per second in Zf brain. We focused our study on endolysosomal vesicle transport in axons of known polarization, separately analyzing kinesin and dynein motor-driven displacements. To validate our assay, we used either loss-of-function mutations of dynein or kinesin 1 or the dynein inhibitor dynapyrazole and quantified several transport parameters. We successfully demonstrated that dynapyrazole reduces the nanoKTP mobile fraction and retrograde run length consistently, while the retrograde run length increased in kinesin 1 mutants. Taking advantage of nanoKTP SHG directional emission, we also quantified fluctuations of vesicle orientation. Thus, by combining endocytosis of nanocrystals having a nonlinear response, fast two-photon microscopy, and high-throughput analysis, we are able to finely monitor fast axonal transport in vivo in the brain of a vertebrate and reveal subtle axonal transport alterations. The high spatiotemporal resolution achieved in our model may be relevant to precisely investigate axonal transport impairment associated with disease models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping