PUBLICATION
Genetically engineered zebrafish as models of skeletal development and regeneration
- Authors
- Henke, K., Farmer, D.T., Niu, X., Kraus, J.M., Galloway, J.L., Youngstrom, D.W.
- ID
- ZDB-PUB-221118-25
- Date
- 2022
- Source
- Bone 167: 116611 (Journal)
- Registered Authors
- Galloway, Jenna, Henke, Katrin
- Keywords
- Development, Genetic engineering, Regeneration, Skeleton, Transgenesis, Zebrafish
- MeSH Terms
-
- Animals
- Bone and Bones
- Cartilage
- Tendons*
- Zebrafish*/genetics
- PubMed
- 36395960 Full text @ Bone
Citation
Henke, K., Farmer, D.T., Niu, X., Kraus, J.M., Galloway, J.L., Youngstrom, D.W. (2022) Genetically engineered zebrafish as models of skeletal development and regeneration. Bone. 167:116611.
Abstract
Zebrafish (Danio rerio) are aquatic vertebrates with significant homology to their terrestrial counterparts. While zebrafish have a centuries-long track record in developmental and regenerative biology, their utility has grown exponentially with the onset of modern genetics. This is exemplified in studies focused on skeletal development and repair. Herein, the numerous contributions of zebrafish to our understanding of the basic science of cartilage, bone, tendon/ligament, and other skeletal tissues are described, with a particular focus on applications to development and regeneration. We summarize the genetic strengths that have made the zebrafish a powerful model to understand skeletal biology. We also highlight the large body of existing tools and techniques available to understand skeletal development and repair in the zebrafish and introduce emerging methods that will aid in novel discoveries in skeletal biology. Finally, we review the unique contributions of zebrafish to our understanding of regeneration and highlight diverse routes of repair in different contexts of injury. We conclude that zebrafish will continue to fill a niche of increasing breadth and depth in the study of basic cellular mechanisms of skeletal biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping