PUBLICATION
A Simple Blood Vessel Imaging Protocol for Live Zebrafish Larva
- Authors
- Vinoth, S., Selvaraj, V., Venkatasubramanian, H., Santhakumar, K.
- ID
- ZDB-PUB-221026-9
- Date
- 2022
- Source
- Zebrafish 19: 177-180 (Journal)
- Registered Authors
- Santhakumar, Kiran
- Keywords
- ImageJ, blood flow, blood vessel, image subtraction, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Larva
- Quercetin*
- Zebrafish*
- PubMed
- 36282957 Full text @ Zebrafish
Citation
Vinoth, S., Selvaraj, V., Venkatasubramanian, H., Santhakumar, K. (2022) A Simple Blood Vessel Imaging Protocol for Live Zebrafish Larva. Zebrafish. 19:177-180.
Abstract
Zebrafish larva is a simple model system to study blood vessel development because of its excellent optical properties. Current methods to study blood vessels in zebrafish involve utilizing either blood vessel-specific transgenic lines or specialized techniques such as microangiography and video capillaroscopy. In this study, we have developed a simple protocol using ImageJ to observe the blood vessels of live zebrafish larva at different developmental stages, namely 1- to 4-day postfertilization (dpf). To validate this protocol, we treated 1 dpf zebrafish embryos with 100 μM quercetin, and analyzed the impaired development of subintestinal vein in the quercetin-treated 3 dpf larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping