PUBLICATION
MiR92b-3p synthetic analogue impairs zebrafish embryonic development, leading to ocular defects, decreased movement and hatching rate, and increased mortality
- Authors
- Kranert, K., Woźny, M., Podlasz, P., Wąsowicz, K., Brzuzan, P.
- ID
- ZDB-PUB-221025-6
- Date
- 2022
- Source
- Journal of applied genetics 64(1): 145-157 (Journal)
- Registered Authors
- Podlasz, Piotr
- Keywords
- Embryogenesis, Gene expression regulation, In vivo transfection, MiR92b, Microinjections, Ocular development
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*/abnormalities
- Embryo, Nonmammalian*/metabolism
- Embryonic Development/genetics
- Larva/genetics
- Larva/metabolism
- Zebrafish*/genetics
- PubMed
- 36274083 Full text @ J. Appl. Genet.
Citation
Kranert, K., Woźny, M., Podlasz, P., Wąsowicz, K., Brzuzan, P. (2022) MiR92b-3p synthetic analogue impairs zebrafish embryonic development, leading to ocular defects, decreased movement and hatching rate, and increased mortality. Journal of applied genetics. 64(1):145-157.
Abstract
The aim of this study was to examine the effect of microRNA 92b-3p (MiR92b-3p) overexpression on the embryonic development of zebrafish. A synthetic MiR92b-3p analogue (mirVana™ mimic, in vivo-ready) was injected at doses up to 5 ng/embryo into the yolk sac of embryos (2-16 cell stage). At 24 h post fertilization (hpf), the locomotor activity of the embryos was measured, and after hatching (72 hpf), the rates of malformation occurrence, hatching, and mortality were determined. Next, the larvae were fixed for histological and molecular examinations. Exposure to the MiR92b-3p mimic impaired embryonic development, leading to increased occurrence of malformations (i.e., pericardial edema, spine curvature, smaller eyes), decreased locomotor activity and hatching rate, and increased mortality. Importantly, the mimic affected retinal differentiation and lens formation during zebrafish embryogenesis, which suggests that MiR92b-3p could be an important factor in the regulation of fish embryogenesis and ocular development. The expression level of MiR92b-3p was substantially higher in the exposed larvae than in the untreated larvae, indicating that the mimic was successfully delivered to the zebrafish. Although screening of potential MiR92b-3p target genes suggested some changes in their expression levels, these results were inconclusive. Together, this study indicates that MiR92b-3p mimic impairs zebrafish embryonic development, and further research is necessary to identify the MiR92b-3p-regulated cell pathways involved in the impairment of the fish's development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping