PUBLICATION
Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae
- Authors
- Shen, C., Cai, Y., Li, J., He, C., Zuo, Z.
- ID
- ZDB-PUB-221002-10
- Date
- 2023
- Source
- Journal of environmental sciences (China) 124: 76-88 (Journal)
- Registered Authors
- He, Chengyong
- Keywords
- Danio rerio, grk1b, mepanipyrim, phototransduction signaling pathway, vision-guided behaviors, visual developmental toxicity
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Fungicides, Industrial*/toxicity
- Larva
- Opsins/metabolism
- Opsins/pharmacology
- Pyrimidines
- Receptors, Aryl Hydrocarbon/metabolism
- Water Pollutants, Chemical*/toxicity
- Zebrafish/metabolism
- PubMed
- 36182181 Full text @ J. Environ. Sci. (China).
Citation
Shen, C., Cai, Y., Li, J., He, C., Zuo, Z. (2023) Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae. Journal of environmental sciences (China). 124:76-88.
Abstract
Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked escape experiments were used to investigate vision-guided behaviors. Meanwhile, transcriptomic sequencing and real-time quantitative PCR assays were applied to assess the potential mechanisms of mepanipyrim-induced visual developmental toxicity and vision-guided behavioral alteration. Our results showed that mepanipyrim exposure could induce retinal impairment and vision-guided behavioral alteration in larval zebrafish. In addition, the grk1b gene of the phototransduction signaling pathway was found to be a potential aryl hydrocarbon receptor (AhR)-regulated gene. Mepanipyrim-induced visual developmental toxicity was potentially related to the AhR signaling pathway. Furthermore, mepanipyrim-induced behavioral alteration was guided by the visual function, and the effects of mepanipyrim on long and middle wavelength light-sensitive opsins may be the main cause of vision-guided behavioral alteration. Our results provide insights into understanding the relationship between visual development and vision-guided behaviors induced by mepanipyrim exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping