PUBLICATION
Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events
- Authors
- Mendieta-Serrano, M.A., Dhar, S., Ng, B.H., Narayanan, R., Lee, J.J.Y., Ong, H.T., Toh, P.J.Y., Röllin, A., Roy, S., Saunders, T.E.
- ID
- ZDB-PUB-220827-42
- Date
- 2022
- Source
- Developmental Cell 57(17): 2095-2110.e5 (Journal)
- Registered Authors
- Dhar, Sunandan, Roy, Sudipto, Saunders, Timothy Edward
- Keywords
- Myomaker, cell elongation, cell migration, fast myocytes, myocyte fusion, slow muscle, zebrafish
- MeSH Terms
-
- Animals
- Muscle Cells*
- Muscle Development
- Muscle, Skeletal/metabolism
- Muscles/metabolism
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 36027918 Full text @ Dev. Cell
Citation
Mendieta-Serrano, M.A., Dhar, S., Ng, B.H., Narayanan, R., Lee, J.J.Y., Ong, H.T., Toh, P.J.Y., Röllin, A., Roy, S., Saunders, T.E. (2022) Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events. Developmental Cell. 57(17):2095-2110.e5.
Abstract
Skeletal myogenesis is dynamic, and it involves cell-shape changes together with cell fusion and rearrangements. However, the final muscle arrangement is highly organized with striated fibers. By combining live imaging with quantitative analyses, we dissected fast-twitch myocyte fusion within the zebrafish myotome in toto. We found a strong mediolateral bias in fusion timing; however, at a cellular scale, there was heterogeneity in cell shape and the relationship between initial position of fast myocytes and resulting fusion partners. We show that the expression of the fusogen myomaker is permissive, but not instructive, in determining the spatiotemporal fusion pattern. Rather, we observed a close coordination between slow muscle rearrangements and fast myocyte fusion. In mutants that lack slow fibers, the spatiotemporal fusion pattern is substantially noisier. We propose a model in which slow muscles guide fast myocytes by funneling them close together, enhancing fusion probability. Thus, despite fusion being highly stochastic, a robust myotome structure emerges at the tissue scale.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping