PUBLICATION
Alcohol Induces Zebrafish Skeletal Muscle Atrophy through HMGB1/TLR4/NF-κB Signaling
- Authors
- Wen, W., Sun, C., Chen, Z., Yang, D., Zhou, Z., Peng, X., Tang, C.
- ID
- ZDB-PUB-220827-23
- Date
- 2022
- Source
- Life (Basel, Switzerland) 12(8): (Journal)
- Registered Authors
- Keywords
- alcoholic myopathy, inflammatory, skeletal muscle atrophy, zebrafish
- MeSH Terms
- none
- PubMed
- 36013390 Full text @ Life (Basel)
Citation
Wen, W., Sun, C., Chen, Z., Yang, D., Zhou, Z., Peng, X., Tang, C. (2022) Alcohol Induces Zebrafish Skeletal Muscle Atrophy through HMGB1/TLR4/NF-κB Signaling. Life (Basel, Switzerland). 12(8).
Abstract
Excessive alcohol consumption can cause alcoholic myopathy, but the molecular mechanism is still unclear. In this study, zebrafish were exposed to 0.5% alcohol for eight weeks to investigate the effect of alcohol on skeletal muscle and its molecular mechanism. The results showed that the body length, body weight, cross-sectional area of the skeletal muscle fibers, Ucrit, and MO2max of the zebrafish were significantly decreased after alcohol exposure. The expression of markers of skeletal muscle atrophy and autophagy was increased, and the expression of P62 was significantly reduced. The content of ROS, the mRNA expression of sod1 and sod2, and the protein expression of Nox2 were significantly increased. In addition, we found that the inflammatory factors Il1β and Tnfα were significantly enriched in skeletal muscle, and the expression of the HMGB1/TLR4/NF-κB signaling axis was also significantly increased. In summary, in this study, we established a zebrafish model of alcohol-induced skeletal muscle atrophy and further elucidated its pathogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping