PUBLICATION
Effects of short-term hypergravity on hematopoiesis and vasculogenesis in embryonic zebrafish
- Authors
- Hariom, S.K., Nelson, E.J.R.
- ID
- ZDB-PUB-220809-17
- Date
- 2022
- Source
- Life sciences in space research 34: 21-29 (Journal)
- Registered Authors
- Keywords
- Gene expression, Hematopoiesis, Hypergravity, Vasculogenesis, Whole-mount in situ hybridization, Zebrafish
- MeSH Terms
-
- Animals
- Hematopoiesis/genetics
- Hypergravity*
- In Situ Hybridization
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 35940686 Full text @ Life Sci Space Res (Amst)
Citation
Hariom, S.K., Nelson, E.J.R. (2022) Effects of short-term hypergravity on hematopoiesis and vasculogenesis in embryonic zebrafish. Life sciences in space research. 34:21-29.
Abstract
Microgravity and hypergravity-induced changes affect both molecular and organismal responses as demonstrated in various animal models. In addition to its inherent advantages, zebrafish have been shown to be incredibly resilient to altered gravity conditions. To understand the effects of altered gravity on animal physiology, especially the cardiovascular system, we used 2 h centrifugations to simulate short-term hypergravity and investigated its effects on zebrafish development. Morphological and in situ hybridization observations show a comparable overall development in both control and treated embryos. Spatiotemporal analysis revealed varied gene expression patterns across different developmental times. Genes driving primitive hematopoiesis (tal1, gata1) and vascular specificity (vegf, etv2) displayed an early onset of expression following hypergravity exposure. Upregulated expression of hematopoiesis-linked genes, such as runx1, cmyb, nos, and pdgf family demonstrate short-term hypergravity to be a factor inducing definitive hematopoiesis through a combinatorial mechanism. We speculate that these short-term hypergravity-induced physiological changes in the developing zebrafish embryos constitute a rescue mechanism to regain homeostasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping