PUBLICATION
Neurotoxicity of tetrabromobisphenol A and SiO2 nanoparticle co-exposure in zebrafish and barrier function of the embryonic chorion
- Authors
- Zhu, B., Lei, L., Fu, K., Zhao, S., Hua, J., Yang, L., Han, J., Li, R., Zhou, B.
- ID
- ZDB-PUB-220720-4
- Date
- 2022
- Source
- The Science of the total environment 845: 157364 (Journal)
- Registered Authors
- Yang, LiHua, Zhou, BingSheng
- Keywords
- Chorion, Co-exposure, Neurotoxicity, SiO(2) nanoparticles, Tetrabromobisphenol A
- MeSH Terms
-
- Acetylcholinesterase/metabolism
- Animals
- Chorion
- Embryo, Nonmammalian
- Larva
- Nanoparticles*/toxicity
- Polybrominated Biphenyls
- Silicon Dioxide/toxicity
- Zebrafish*
- PubMed
- 35843329 Full text @ Sci. Total Environ.
Citation
Zhu, B., Lei, L., Fu, K., Zhao, S., Hua, J., Yang, L., Han, J., Li, R., Zhou, B. (2022) Neurotoxicity of tetrabromobisphenol A and SiO2 nanoparticle co-exposure in zebrafish and barrier function of the embryonic chorion. The Science of the total environment. 845:157364.
Abstract
Silicon dioxide nanoparticles (n-SiO2) absorb tetrabromobisphenol A (TBBPA) and modify its bioavailability and toxicity in the aquatic phase; embryonic chorion is an efficient barrier against nanoparticles (e.g., SiO2) and influences their toxicity. However, few studies have investigated developmental neurotoxicity in fish after co-exposure to TBBPA and n-SiO2, especially considering the barrier function of the chorion. In the present study, zebrafish embryos were exposed to TBBPA (50, 100, and 200 μg/L) alone or in combination with n-SiO2 (25 mg/L) until 24 or 120 h post fertilization (hpf), in the presence and absence of the chorion. The results confirmed that TBBPA exposure alone significantly downregulated the expression of neurodevelopment marker genes (mbp, alpha-tubulin, shha, and gfap), altered acetylcholinesterase activity and acetylcholine content, and affected locomotor behavior at different developmental stages. Moreover, the results indicated that n-SiO2 promoted TBBPA-induced neurotoxic effects in zebrafish larvae at 120 hpf, including further repression of the transcription of CNS-related genes, disruption of the cholinergic system, and decrease in the average swimming speed under dark/light stimulation. However, scanning electron microscopy/energy dispersive spectroscopy analysis revealed that at 24 hpf, the embryonic chorion efficiently blocked n-SiO2 and consequently decreased the bioaccumulation of TBBPA and TBBPA-induced neurotoxicity in dechorionated zebrafish embryos. Taken together, the results demonstrate that n-SiO2 affected the bioavailability and neurodevelopmental toxicity of TBBPA, and their combined toxicity to zebrafish embryos was mitigated by embryonic chorion, which will facilitate risk assessment on n-SiO2 and TBBPA and improve understanding the function of the fish embryonic chorion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping