PUBLICATION
Participation of ecto-5'-nucleotidase in the inflammatory response in an adult zebrafish (Danio rerio) model
- Authors
- Nazario, L.R., de Sousa, J.S., de Moraes Silveira, F.S., Costa, K.M., de Oliveira, G.M.T., Bogo, M.R., da Silva, R.S.
- ID
- ZDB-PUB-220703-5
- Date
- 2022
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 260: 109402 (Journal)
- Registered Authors
- Keywords
- Adenosine, CD75, Endotoxin, Lipopolysaccharide, Purinergic system
- MeSH Terms
-
- 5'-Nucleotidase*/genetics
- 5'-Nucleotidase*/metabolism
- Adenosine/metabolism
- Animals
- Lipopolysaccharides/pharmacology
- Tumor Necrosis Factor-alpha/genetics
- Zebrafish*/metabolism
- PubMed
- 35779837 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Nazario, L.R., de Sousa, J.S., de Moraes Silveira, F.S., Costa, K.M., de Oliveira, G.M.T., Bogo, M.R., da Silva, R.S. (2022) Participation of ecto-5'-nucleotidase in the inflammatory response in an adult zebrafish (Danio rerio) model. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 260:109402.
Abstract
The ecto-5'-nucleotidase is an important source of adenosine in the extracellular medium. Adenosine modulation appears early in evolution and performs several biological functions, including a role as an anti-inflammatory molecule. Here, we evaluate the activity and mRNA expression of ecto-5'-nucleotidase in response to lipopolysaccharide (LPS) using zebrafish as a model. Adult zebrafish were injected with LPS (10 μg/g). White blood cell differential counts, inflammatory markers, and ecto-5'-nucleotidase activity and expression in the encephalon, kidney, heart, and intestine were evaluated at 2, 12, and 24 h post-injection (hpi). At 2 hpi of LPS, an increase in neutrophils and monocytes in peripheral blood was observed, which was accompanied by increased tnf-α expression in the heart, kidney, and encephalon, and increased cox-2 expression in the intestine and kidney. At 12 hpi, monocytes remained elevated in the peripheral blood, while tnf-α expression was also increased in the intestine. At 24 hpi, the white blood cell differential count no longer differed from that of the control, whereas tnf-α expression remained elevated in the encephalon but reduced in the kidney compared with the controls. AMP hydrolysis in LPS-treated animals was increased in the heart at 24 hpi [72 %; p = 0.029] without affecting ecto-5'-nucleotidase gene expression. These data indicate that, in most tissues studied, inflammation does not affect ecto-5'-nucleotidase activity, whereas in the heart, a delayed increase in ecto-5'-nucleotidase activity could be related to tissue repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping