PUBLICATION
Adverse effects of SYP-3343 on zebrafish development via ROS-mediated mitochondrial dysfunction
- Authors
- Chen, G., Wang, M., Zhu, P., Wang, G., Hu, T.
- ID
- ZDB-PUB-220625-32
- Date
- 2022
- Source
- Journal of hazardous materials 437: 129382 (Journal)
- Registered Authors
- Wang, Guixue
- Keywords
- Apoptosis, Mitochondria, Reactive oxygen species, SYP-3343, Zebrafish
- Datasets
- GEO:GSE224231
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Apoptosis
- Embryo, Nonmammalian*
- Mitochondria
- Oxidative Stress
- Reactive Oxygen Species/metabolism
- Zebrafish*/metabolism
- PubMed
- 35749898 Full text @ J. Hazard. Mater.
Citation
Chen, G., Wang, M., Zhu, P., Wang, G., Hu, T. (2022) Adverse effects of SYP-3343 on zebrafish development via ROS-mediated mitochondrial dysfunction. Journal of hazardous materials. 437:129382.
Abstract
As a newly-invented and highly-efficiency strobilurin fungicide, pyraoxystrobin (SYP-3343) has been recognized as a highly poisonous toxin for a variety of aquatic organisms. Nevertheless, the developmental toxicity and potential mechanism of SYP-3343 have not been well-documented. The results showed that SYP-3343 was relatively stable and maintained within the range of 20 % in 24 h, and the LC50 value to embryos at 72 hpf was 17.13 μg/L. The zebrafish embryotoxicity induced by 1, 2, 4, and 8 μg/L SYP-3343 is demonstrated by repressive embryo incubation, enhancive mortality rate, abnormal heart rate, malformed morphological characteristic, and impaired spontaneous coiling, indicating SYP-3343 mostly exerted its toxicity in a dose- and time-dependent manner. Besides SYP-3343 was critically involved in regulating cell cycle, mitochondrial membrane potential, and reactive oxygen species production as well as zebrafish primary cells apoptosis, which can be mitigated using antioxidant N-acetyl-L-cysteine. A significant change occurred in total protein content, the biochemical indices, and antioxidant capacities owing to SYP-3343 exposure. Additionally, SYP-3343 altered the mRNA levels of heart development-, mitochondrial function-, and apoptosis-related genes in zebrafish embryos. These results indicated that SYP-3343 induced apoptosis accompanying reactive oxygen species-initiated mitochondrial dysfunction in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping