PUBLICATION
NMDA receptor antagonist MK801 reduces dendritic spine density and stability in zebrafish pyramidal neurons
- Authors
- Plata, A.L.D., Robles, E.
- ID
- ZDB-PUB-220622-42
- Date
- 2022
- Source
- Neuroscience 498: 50-63 (Journal)
- Registered Authors
- Keywords
- Activity-dependent, Development, Neuronal morphology, Visual system
- MeSH Terms
-
- Animals
- Dendrites
- Dendritic Spines
- Dizocilpine Maleate
- Pyramidal Cells
- Receptors, N-Methyl-D-Aspartate*
- Zebrafish*
- PubMed
- 35718218 Full text @ Neuroscience
Citation
Plata, A.L.D., Robles, E. (2022) NMDA receptor antagonist MK801 reduces dendritic spine density and stability in zebrafish pyramidal neurons. Neuroscience. 498:50-63.
Abstract
NMDA-type glutamate receptors play a critical role in activity-dependent neurite growth. We employed cell type-specific genetic labeling in zebrafish to examine the effects of NMDA receptor antagonism on the morphological development of tectal pyramidal neurons (PyrNs). Our data demonstrate that the NMDA receptor antagonist MK801 reduces PyrN spine density and stability without significantly altering dendritic growth and branching. However, the axons that synapse onto PyrN dendritic spines do exhibit reduced arbor growth and branching in response to MK801 treatment. Axons that synapse with PyrNs, but not on spines, are unaffected by MK801 treatment. These findings may reflect different roles for NMDARs during the development of spiny and aspiny dendrites.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping