PUBLICATION
Fusobacterium nucleatum mediates endothelial damage and increased permeability following single species and polymicrobial infection
- Authors
- Farrugia, C., Stafford, G.P., Gains, A.F., Cutts, A.R., Murdoch, C.
- ID
- ZDB-PUB-220602-5
- Date
- 2022
- Source
- Journal of periodontology 93(9): 1421-1433 (Journal)
- Registered Authors
- Keywords
- atherosclerosis, cardiovascular diseases, host microbial interactions, periodontitis, porphyromonas gingivalis
- MeSH Terms
-
- Animals
- Coinfection*
- Dextrans/pharmacology
- Endothelium
- Fusobacterium Infections*
- Fusobacterium nucleatum
- Periodontitis*/microbiology
- Permeability
- Platelet Endothelial Cell Adhesion Molecule-1
- Porphyromonas gingivalis
- Virulence Factors
- Zebrafish
- PubMed
- 35644006 Full text @ J Periodontol
Citation
Farrugia, C., Stafford, G.P., Gains, A.F., Cutts, A.R., Murdoch, C. (2022) Fusobacterium nucleatum mediates endothelial damage and increased permeability following single species and polymicrobial infection. Journal of periodontology. 93(9):1421-1433.
Abstract
Background Numerous lines of evidence link periodontal pathobionts and their virulence factors with endothelial damage. Most research has been conducted using single species infections at the exclusion of other periodontal microorganisms that have been identified in vascular tissue. Here we assessed endothelial infection with either single or mixed periodontal species infection and examined their effect on endothelial damage and permeability.
Methods Cell surface abundance of PECAM-1 or endothelial permeability following infection with Porphyromonas gingivalis, Fusobacterium nucleatum subspecies (ssp) nucleatum, ssp polymorphum or Tannerella forsythia as single or mixed species infection was determined by flow cytometry and a fluorescent dextran permeability assay. Zebrafish embryos were infected systemically with either single or mixed species with mortality and disease measured overtime.
Results F. nucleatum ssp nucleatum, ssp polymorphum and P. gingivalis significantly reduced PECAM-1 abundance in single species infection, whereas T. forsythia had no effect. F. nucleatum ssp polymorphum caused considerable mortality and morbidity in a zebrafish systemic infection model. Polymicrobial infection underscored the virulence of F. nucleatum ssp polymorphum in particular with increased endothelial cell death and reduced PECAM-1 abundance in co-infection studies with this organism. When injected systemically into zebrafish in polymicrobial infection, fluorescently-labelled bacteria were distributed throughout the vasculature and cardiac region where, in some instances, they co-localised with each other.
Conclusion These data provide further evidence on the effects of F. nucleatum on endothelium adhesion molecule abundance and permeability while also highlighting the importance of performing polymicrobial infection to study the molecular mechanisms associated with periodontal pathogen-induced vascular damage. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping