PUBLICATION

Generation of specialized blood vessels via lymphatic transdifferentiation

Authors
Das, R.N., Tevet, Y., Safriel, S., Han, Y., Moshe, N., Lambiase, G., Bassi, I., Nicenboim, J., Brückner, M., Hirsch, D., Eilam-Altstadter, R., Herzog, W., Avraham, R., Poss, K.D., Yaniv, K.
ID
ZDB-PUB-220527-2
Date
2022
Source
Nature   606(7914): 570-575 (Journal)
Registered Authors
Han, Yanchao, Herzog, Wiebke, Poss, Kenneth D., Yaniv, Karina
Keywords
none
Datasets
GEO:GSE197161
MeSH Terms
  • Animal Fins/cytology
  • Animals
  • Blood Vessels*/cytology
  • Cell Lineage
  • Cell Transdifferentiation*
  • Endothelial Cells/cytology
  • Lymphatic Vessels*/cytology
  • Zebrafish
PubMed
35614218 Full text @ Nature
Abstract
The lineage and developmental trajectory of a cell are key determinants of cellular identity. In the vascular system, endothelial cells (ECs) of blood and lymphatic vessels differentiate and specialize to cater to the unique physiological demands of each organ1,2. Although lymphatic vessels were shown to derive from multiple cellular origins, lymphatic ECs (LECs) are not known to generate other cell types3,4. Here we use recurrent imaging and lineage-tracing of ECs in zebrafish anal fins, from early development to adulthood, to uncover a mechanism of specialized blood vessel formation through the transdifferentiation of LECs. Moreover, we demonstrate that deriving anal-fin vessels from lymphatic versus blood ECs results in functional differences in the adult organism, uncovering a link between cell ontogeny and functionality. We further use single-cell RNA-sequencing analysis to characterize the different cellular populations and transition states involved in the transdifferentiation process. Finally, we show that, similar to normal development, the vasculature is rederived from lymphatics during anal-fin regeneration, demonstrating that LECs in adult fish retain both potency and plasticity for generating blood ECs. Overall, our research highlights an innate mechanism of blood vessel formation through LEC transdifferentiation, and provides in vivo evidence for a link between cell ontogeny and functionality in ECs.
Genes / Markers
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Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes