PUBLICATION
Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos
- Authors
- Ibarra, B.A., Jiang, X., Treffy, R.W., Saxena, A.
- ID
- ZDB-PUB-220520-4
- Date
- 2022
- Source
- STAR protocols 3: 101380 (Journal)
- Registered Authors
- Saxena, Ankur
- Keywords
- Cancer, Developmental biology, Microscopy, Model Organisms, Stem Cells
- MeSH Terms
-
- Animals
- Cell Tracking
- Humans
- Neural Crest*
- Neuroblastoma*
- Tumor Microenvironment
- Zebrafish
- PubMed
- 35586316 Full text @ STAR Protoc
Citation
Ibarra, B.A., Jiang, X., Treffy, R.W., Saxena, A. (2022) Injection of human neuroblastoma cells into neural crest streams in live zebrafish embryos. STAR protocols. 3:101380.
Abstract
Cancer cell behavior is highly microenvironment dependent, but we have a limited understanding of malignant cell-microenvironment interactions in vivo. Here, we describe a protocol for xenotransplanting human neuroblastoma (NB) cells into streams of migrating neural crest stem cells in zebrafish embryos, followed by confocal time-lapse imaging and cell tracking. This high-resolution model system facilitates the quantitative spatiotemporal analysis of cancer cell-cell and cell-environment interactions. For complete details on the use and execution of this protocol, please refer to Treffy et al. (2021).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping