PUBLICATION
Hypoxia regulates cytokines expression and neutrophils migration by ERK signaling in zebrafish
- Authors
- He, S.M., Sun, S., Chen, A.Q., Lv, S.J., Qiu, C.Z., Wei, M.L., Liu, W., Liu, H.R., Zhang, L., Ren, D.L.
- ID
- ZDB-PUB-220517-4
- Date
- 2022
- Source
- Fish & shellfish immunology 125: 212-219 (Journal)
- Registered Authors
- Keywords
- Cytokines, ERK, Hypoxia, Neutrophils, Zebrafish
- MeSH Terms
-
- Animals
- Cytokines*/genetics
- Cytokines*/metabolism
- Hypoxia/genetics
- Inflammation/chemically induced
- Inflammation/genetics
- Neutrophils
- Oxygen/metabolism
- Water
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 35569778 Full text @ Fish Shellfish Immunol.
Citation
He, S.M., Sun, S., Chen, A.Q., Lv, S.J., Qiu, C.Z., Wei, M.L., Liu, W., Liu, H.R., Zhang, L., Ren, D.L. (2022) Hypoxia regulates cytokines expression and neutrophils migration by ERK signaling in zebrafish. Fish & shellfish immunology. 125:212-219.
Abstract
Normal dissolved oxygen in water is essential for maintaining the physiological functions of fish, but environmental pollution, such as eutrophication can lead to a decrease in oxygen content in water. How this reduction of dissolved oxygen in water affects the immune functions of fish and the potential regulatory mechanisms have not been thoroughly elucidated. In this study, we made full use of the aquatic model animal zebrafish to explore this question. In a model of LPS-induced inflammation, we found that hypoxia induced by infusing nitrogen into water increased the expression of pro-inflammatory cytokines, such as il-1β, il-6, and il-8. In vivo imaging also showed that hypoxia significantly increased neutrophil migration to the site of caudal fin injury in the transgenic line. Subsequently, we found that the phosphorylation level of ERK protein was significantly activated upon hypoxia and proved the roles of ERK signaling in the expression of pro-inflammatory cytokines and neutrophil migration in zebrafish. This study indicated that reduced water oxygen significantly increases the inflammatory response of the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping