PUBLICATION
Mapping Molecular Networks within Clitoria ternatea Linn. against LPS-Induced Neuroinflammation in Microglial Cells, with Molecular Docking and In Vivo Toxicity Assessment in Zebrafish
- Authors
- Mat Zian, N.F.A., Swain, P., Mohd Faudzi, S.M., Zakaria, N., Wan Ibrahim, W.N., Abu Bakar, N., Shaari, K., Stanslas, J., Choi, T.I., Kim, C.H.
- ID
- ZDB-PUB-220424-8
- Date
- 2022
- Source
- Pharmaceuticals (Basel, Switzerland) 15(4): (Journal)
- Registered Authors
- Kim, Cheol-Hee
- Keywords
- Alzheimer’s disease, Clitoria ternatea, anti-neuroinflammation, butterfly pea, molecular networking, zebrafish toxicity
- MeSH Terms
- none
- PubMed
- 35455463 Full text @ Pharmaceuticals (Basel)
Citation
Mat Zian, N.F.A., Swain, P., Mohd Faudzi, S.M., Zakaria, N., Wan Ibrahim, W.N., Abu Bakar, N., Shaari, K., Stanslas, J., Choi, T.I., Kim, C.H. (2022) Mapping Molecular Networks within Clitoria ternatea Linn. against LPS-Induced Neuroinflammation in Microglial Cells, with Molecular Docking and In Vivo Toxicity Assessment in Zebrafish. Pharmaceuticals (Basel, Switzerland). 15(4):.
Abstract
Clitoria ternatea Linn. (CT), or butterfly pea, is an Ayurvedic plant traditionally used as a brain tonic. Recently, it was reported to be of use in treating central nervous system (CNS) disorders, i.e., as an antistress treatment and antidepressant. In the present study, we report a detailed phytochemical profile of the ethyl acetate fraction of the flower of CT (CTF_EA) with significant neuroprotective and anti-neuroinflammatory properties in both LPS-activated BV-2 and SK-N-SH cells. Concurrently, the molecular network (MN) derived from the CTF_EA metabolome allows putative identification of flavonol 3-O-glycosides, hydrocinnamic acids, and primary metabolites. Molecular docking studies suggest that CTF_EA preferentially targets iNOS, resulting in a decrease in nitric oxide (NO). Furthermore, no toxic effects on normal embryonic development, blood vessel formation, and apoptosis are observed when CTF_EA is tested for in vivo toxicity in zebrafish models. The overall preliminary results suggest the anti-neuroinflammatory and neuroprotective effects of CT and provide scientific support for the efficacy of this medicinal plant at local and traditional levels. However, studies on the targeted isolation of bioactive metabolites, in-depth pharmacological efficacy, and safety in mammalian models are urgently needed to expand our understanding of this plant before it is developed into a promising therapeutic agent for brain-related diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping