PUBLICATION
PurA facilitates Edwardsiella piscicida to escape NF-κB signaling activation
- Authors
- Hu, F., Zhang, Y., Liu, Q., Wang, Z.
- ID
- ZDB-PUB-220409-17
- Date
- 2022
- Source
- Fish & shellfish immunology 124: 254-260 (Journal)
- Registered Authors
- Keywords
- Adenylosuccinate synthase, Edwardsiella piscicida, Immuno-evasion, NF-κB singlaing
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics
- Edwardsiella*/genetics
- Enterobacteriaceae Infections*/microbiology
- Enterobacteriaceae Infections*/veterinary
- Fish Diseases*/microbiology
- NF-kappa B/genetics
- Signal Transduction
- Zebrafish/genetics
- PubMed
- 35395412 Full text @ Fish Shellfish Immunol.
Citation
Hu, F., Zhang, Y., Liu, Q., Wang, Z. (2022) PurA facilitates Edwardsiella piscicida to escape NF-κB signaling activation. Fish & shellfish immunology. 124:254-260.
Abstract
The host NF-κB signaling pathway plays critical role in defensing against bacterial infection. However, bacteria also evolve strategies to escape from host clearance. Edwardsiella piscicida is a threatening pathogen in aquaculture, while the molecular mechanism of E. piscicida in inhibiting NF-κB signaling remains largely unknown. Herein, using E. piscicida transposon insertion mutant library combined with a NF-κB luciferase reporter system, we identified forty-six genes of E. piscicida, which were involved in inhibiting the NF-κB signaling activation in vitro. Moreover, we further explored the top 10 significantly changed mutants through zebrafish larvae infection model and validated that six genes were involved in inhibiting NF-κB activation in vivo. Specifically, we identified the adenylosuccinate synthase mutated strain (ΔpurA) infection exhibited a robust activation of NF-κB signaling, along with higher expression of cxcl8a and cxcl8b to mediate the recruitment of neutrophils in vivo. Taken together, these results identified the key factors of E. piscicida in inhibiting NF-κB activation, which will contribute to better understanding the pathogenesis of this important pathogen.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping