PUBLICATION
Nano-bubble hydrogen water: An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model
- Authors
- Li, C., Cao, Y., Kohei, F., Hao, H., Peng, G., Cheng, C., Ye, J.
- ID
- ZDB-PUB-220308-8
- Date
- 2022
- Source
- Virologica Sinica 37(2): 277-283 (Journal)
- Registered Authors
- Keywords
- Inflammation, Nano-bubble hydrogen water (nano-HW), Spring viraemia of carp virus (SVCV), Zebrafish
- MeSH Terms
-
- Animals
- Carps*
- Fish Diseases*/drug therapy
- Hydrogen/pharmacology
- Hydrogen/therapeutic use
- Inflammation/drug therapy
- Rhabdoviridae
- Rhabdoviridae Infections*/drug therapy
- Water
- Zebrafish
- PubMed
- 35249853 Full text @ Virol Sin
Citation
Li, C., Cao, Y., Kohei, F., Hao, H., Peng, G., Cheng, C., Ye, J. (2022) Nano-bubble hydrogen water: An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model. Virologica Sinica. 37(2):277-283.
Abstract
Since the anti-inflammatory effect of hydrogen has been widely known, it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory reactions. However, hydrogen is slightly soluble in water, which leads to poor effect of oral hydrogen-rich water therapy. In this study, the nano-bubble hydrogen water (nano-HW) (about 0.7 ppm) was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus (SVCV)-infected zebrafish as model. Three-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group (control group). The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40% after treatment with nano-bubble hydrogen water, and qRT-PCR results showed that SVCV replication was significantly inhibited. Histopathological examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen water. Futhermore, SVCV infection caused reactive oxygen species (ROS) accumulation was significantly reduced upon nano-HW treatment. The level of proinflammatory cytokines IL-1β, IL-8, and TNF-α was remarkably reduced in the nano-HW-treated group in vivo and in vitro. Taken together, our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish, which suggests that nano-HW can be applied to antiviral research，and provides a novel therapeutic strategy for virus-caused inflammation related disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping