PUBLICATION
Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish
- Authors
- Corbacho, J., Sanabria-Reinoso, E., Buono, L., Fernández-Miñan, A., Martínez-Morales, J.R.
- ID
- ZDB-PUB-220219-5
- Date
- 2022
- Source
- Frontiers in cell and developmental biology 9: 817191 (Review)
- Registered Authors
- Martinez-Morales, Juan R.
- Keywords
- Gal4/UAS, TRAP, enhancer-trap, translatome, zebrafish
- Datasets
- GEO:GSE192511
- MeSH Terms
- none
- PubMed
- 35174174 Full text @ Front Cell Dev Biol
Citation
Corbacho, J., Sanabria-Reinoso, E., Buono, L., Fernández-Miñan, A., Martínez-Morales, J.R. (2022) Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish. Frontiers in cell and developmental biology. 9:817191.
Abstract
Developmental and physiological processes depend on the transcriptional and translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations. Here we combined the TRAP approach with adapted enhancer trap methods (trap-TRAP) to systematically generate zebrafish transgenic lines suitable for tissue-specific translatome interrogation. Through the random integration of a GFP-tagged version of the large subunit ribosomal protein L10a (EGFP-Rpl10a), we have generated stable lines driving expression in a variety of tissues, including the retina, skeletal muscle, lateral line primordia, rhombomeres, or jaws. To increase the range of applications, a UAS:TRAP transgenic line compatible with available Gal4 lines was also generated and tested. The resulting collection of lines and applications constitutes a resource for the zebrafish community in developmental genetics, organ physiology and disease modelling.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping