PUBLICATION
Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System
- Authors
- Henry, J., Bai, Y., Kreuder, F., Mawdsley, D., Kaslin, J., Wlodkowic, D.
- ID
- ZDB-PUB-220202-27
- Date
- 2022
- Source
- Zebrafish 19(1): 32-35 (Journal)
- Registered Authors
- Kaslin, Jan, Mawdsley, David
- Keywords
- array, automated, behavior, ecotoxicology, embryo, neurotoxicity, screening, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*/drug effects
- Nervous System/drug effects*
- Toxicity Tests*
- Zebrafish*/physiology
- PubMed
- 35100037 Full text @ Zebrafish
Citation
Henry, J., Bai, Y., Kreuder, F., Mawdsley, D., Kaslin, J., Wlodkowic, D. (2022) Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System. Zebrafish. 19(1):32-35.
Abstract
Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack of effective embryo in-test positioning, wide-field imaging, and high-throughput bioinformatic analytics. In this study, we demonstrate advantages of using custom large-density embryo arrays in conjunction with an open-source ultra-high-definition video imaging system. Moreover, we present a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of specimens in photomotor response bioassays. The system validation was showcased in a proof-of-concept neurotoxicity analysis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping