PUBLICATION
Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography
- Authors
- Lichtenegger, A., Mukherjee, P., Tamaoki, J., Bian, L., Zhu, L., El-Sadek, I.A., Makita, S., Leskovar, K., Kobayashi, M., Baumann, B., Yasuno, Y.
- ID
- ZDB-PUB-220126-25
- Date
- 2022
- Source
- Journal of Biomedical Optics 27(1): (Journal)
- Registered Authors
- Bian, Lixuan, Kobayashi, Makoto, Tamaoki, Junya
- Keywords
- Jones matrix OCT, non-destructive, polarization-sensitive, zebrafish
- MeSH Terms
-
- Animals
- Birefringence
- Refraction, Ocular
- Tomography, Optical Coherence*
- Zebrafish*
- PubMed
- 35064657 Full text @ J. Biomed. Opt.
Citation
Lichtenegger, A., Mukherjee, P., Tamaoki, J., Bian, L., Zhu, L., El-Sadek, I.A., Makita, S., Leskovar, K., Kobayashi, M., Baumann, B., Yasuno, Y. (2022) Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography. Journal of Biomedical Optics. 27(1):.
Abstract
Significance The scattering and polarization characteristics of various organs of in vivo wildtype zebrafish in three development stages were investigated using a non-destructive and label-free approach. The presented results showed a promising first step for the usability of Jones-matrix optical coherence tomography (JM-OCT) in zebrafish-based research.
Aim We aim to visualize and quantify the scatter and polarization signatures of various zebrafish organs for larvae, juvenile, and young adult animals in vivo in a non-invasive and label-free way.
Approach A custom-built polarization-sensitive JM-OCT setup in combination with a motorized translation stage was utilized to investigate live zebrafish. Depth-resolved scattering (intensity and attenuation coefficient) and polarization (birefringence and degree of polarization uniformity) properties were analyzed. OCT angiography (OCT-A) was utilized to investigate the vasculature label-free and non-destructively.
Results The scatter and polarization signatures of the zebrafish organs such as the eye, gills, and muscles were investigated. The attenuation coefficient and birefringence changes between 1- and 2-month-old animals were evaluated in selected organs. OCT-A revealed the vasculature of in vivo larvae and juvenile zebrafish in a label-free manner.
Conclusions JM-OCT offers a rapid, label-free, non-invasive, tissue specific, and three-dimensional imaging tool to investigate in vivo processes in zebrafish in various development stages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping