PUBLICATION
Myocardial Afterload Is a Key Biomechanical Regulator of Atrioventricular Myocyte Differentiation in Zebrafish
- Authors
- Ahuja, N., Ostwald, P., Gendernalik, A., Guzzolino, E., Pitto, L., Bark, D., Garrity, D.M.
- ID
- ZDB-PUB-220121-10
- Date
- 2022
- Source
- Journal of cardiovascular development and disease 9(1): (Journal)
- Registered Authors
- Garrity, Deborah
- Keywords
- afterload, biomechanics, developmental biology, heart, hemodynamics, valve, zebrafish
- MeSH Terms
- none
- PubMed
- 35050232 Full text @ J Cardiovasc Dev Dis
Citation
Ahuja, N., Ostwald, P., Gendernalik, A., Guzzolino, E., Pitto, L., Bark, D., Garrity, D.M. (2022) Myocardial Afterload Is a Key Biomechanical Regulator of Atrioventricular Myocyte Differentiation in Zebrafish. Journal of cardiovascular development and disease. 9(1):.
Abstract
Heart valve development is governed by both genetic and biomechanical inputs. Prior work has demonstrated that oscillating shear stress associated with blood flow is required for normal atrioventricular (AV) valve development. Cardiac afterload is defined as the pressure the ventricle must overcome in order to pump blood throughout the circulatory system. In human patients, conditions of high afterload can cause valve pathology. Whether high afterload adversely affects embryonic valve development remains poorly understood. Here we describe a zebrafish model exhibiting increased myocardial afterload, caused by vasopressin, a vasoconstrictive drug. We show that the application of vasopressin reliably produces an increase in afterload without directly acting on cardiac tissue in zebrafish embryos. We have found that increased afterload alters the rate of growth of the cardiac chambers and causes remodeling of cardiomyocytes. Consistent with pathology seen in patients with clinically high afterload, we see defects in both the form and the function of the valve leaflets. Our results suggest that valve defects are due to changes in atrioventricular myocyte signaling, rather than pressure directly acting on the endothelial valve leaflet cells. Cardiac afterload should therefore be considered a biomechanical factor that particularly impacts embryonic valve development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping