PUBLICATION
Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads
- Authors
- Souchaud, A., Boutillon, A., Charron, G., Asnacios, A., Nous, C., David, N.B., Graner, F., Gallet, F.
- ID
- ZDB-PUB-220115-2
- Date
- 2022
- Source
- Development (Cambridge, England) 149(4): (Journal)
- Registered Authors
- Keywords
- Cell aggregate, Elastic gel, Mechanical stress, PDMS, Sensor, Zebrafish
- MeSH Terms
-
- Animals
- Cell Aggregation
- Cell Culture Techniques/instrumentation
- Cell Culture Techniques/methods
- Cell Line, Tumor
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Imaging, Three-Dimensional/methods*
- Mice
- Microscopy, Fluorescence, Multiphoton
- Shear Strength*
- Zebrafish
- PubMed
- 35029679 Full text @ Development
Citation
Souchaud, A., Boutillon, A., Charron, G., Asnacios, A., Nous, C., David, N.B., Graner, F., Gallet, F. (2022) Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads. Development (Cambridge, England). 149(4):.
Abstract
To investigate the role of mechanical constraints in morphogenesis and development, we develop a pipeline of techniques based on incompressible elastic sensors. These techniques combine the advantages of incompressible liquid droplets, which have been used as precise in situ shear stress sensors, and of elastic compressible beads, which are easier to tune and to use. Droplets of a polydimethylsiloxane (PDMS) mix, made fluorescent through specific covalent binding to a rhodamin dye, are produced by a microfluidics device. The elastomer rigidity after polymerization is adjusted to the tissue rigidity. Its mechanical properties are carefully calibrated in situ, for a sensor embedded in a cell aggregate submitted to uniaxial compression. Thelocal shear stress tensor is retrieved from the sensor shape, accurately reconstructed through an active contour method. In vitro, within cell aggregates, and in vivo, in the prechordal plate of the Zebrafish embryo during gastrulation,our pipeline of techniques demonstrates its efficiency to directly measure the three dimensional shear stress repartition within a tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping