PUBLICATION
Aquatic surface respiration improves survival during hypoxia in zebrafish (Danio rerio) lacking hypoxia-inducible factor 1-α
- Authors
- Mandic, M., Flear, K., Qiu, P., Pan, Y.K., Perry, S.F., Gilmour, K.M.
- ID
- ZDB-PUB-220113-4
- Date
- 2022
- Source
- Proceedings. Biological sciences 289: 20211863 (Journal)
- Registered Authors
- Perry, Steve F.
- Keywords
- aquatic surface respiration, early development, hypoxia-inducible factor-1α, intermittent hypoxia, survival, zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Regulation
- Hypoxia*
- Larva/physiology
- Respiration
- Zebrafish*/physiology
- PubMed
- 35016541 Full text @ Proc. Biol. Sci.
Citation
Mandic, M., Flear, K., Qiu, P., Pan, Y.K., Perry, S.F., Gilmour, K.M. (2022) Aquatic surface respiration improves survival during hypoxia in zebrafish (Danio rerio) lacking hypoxia-inducible factor 1-α. Proceedings. Biological sciences. 289:20211863.
Abstract
Hypoxia-inducible factor 1-α (Hif-1α), an important transcription factor regulating cellular responses to reductions in O2, previously was shown to improve hypoxia tolerance in zebrafish (Danio rerio). Here, we examined the contribution of Hif-1α to hypoxic survival, focusing on the benefit of aquatic surface respiration (ASR). Wild-type and Hif-1α knockout lines of adult zebrafish were exposed to two levels (moderate or severe) of intermittent hypoxia. Survival was significantly compromised in Hif-1α knockout zebrafish prevented from accessing the surface during severe (16 mmHg) but not moderate (23 mmHg) hypoxia. When allowed access to the surface in severe hypoxia, survival times did not differ between wild-type and Hif-1α knockouts. Performing ASR mitigated the negative effects of the loss of Hif-1α with the knockouts initiating ASR at a higher PO2 threshold and performing ASR for longer than wild-types. The loss of Hif-1α had little impact on survival in fish between 1 and 5 days post-fertilization, but as the larvae aged, their reliance on Hif-1α increased. Similar to adult fish, ASR compensated for the loss of Hif-1α on survival. Together, these results demonstrate that age, hypoxia severity and, in particular, the ability to perform ASR significantly modulate the impact of Hif-1α on survival in hypoxic zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping