PUBLICATION
Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale
- Authors
- Wu, J., Lu, Z., Jiang, D., Guo, Y., Qiao, H., Zhang, Y., Zhu, T., Cai, Y., Zhang, X., Zhanghao, K., Xie, H., Yan, T., Zhang, G., Li, X., Jiang, Z., Lin, X., Fang, L., Zhou, B., Xi, P., Fan, J., Yu, L., Dai, Q.
- ID
- ZDB-PUB-220106-9
- Date
- 2021
- Source
- Cell 184: 3318-3332.e17 (Journal)
- Registered Authors
- Keywords
- adaptive optics, calcium imaging, intravital, light-field microscopy, long-term high-speed imaging, migrasome, phototoxicity, retraction fiber, tumor metastasis
- MeSH Terms
-
- Algorithms*
- Animals
- Calcium/metabolism
- Cell Line, Tumor
- Cell Membrane/metabolism
- Cell Movement
- Drosophila
- HeLa Cells
- Humans
- Imaging, Three-Dimensional*
- Larva/physiology
- Liver/diagnostic imaging
- Male
- Mice, Inbred C57BL
- Neoplasms/pathology
- Optics and Photonics*
- Rats, Sprague-Dawley
- Signal-To-Noise Ratio
- Subcellular Fractions/physiology
- Time Factors
- Tomography*
- Zebrafish
- PubMed
- 34038702 Full text @ Cell
Citation
Wu, J., Lu, Z., Jiang, D., Guo, Y., Qiao, H., Zhang, Y., Zhu, T., Cai, Y., Zhang, X., Zhanghao, K., Xie, H., Yan, T., Zhang, G., Li, X., Jiang, Z., Lin, X., Fang, L., Zhou, B., Xi, P., Fan, J., Yu, L., Dai, Q. (2021) Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale. Cell. 184:3318-3332.e17.
Abstract
Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular behaviors and organelle functions during native physiological processes. However, optical heterogeneity, tissue opacity, and phototoxicity pose great challenges. Here, we propose a computational imaging framework, termed digital adaptive optics scanning light-field mutual iterative tomography (DAOSLIMIT), featuring high-speed, high-resolution 3D imaging, tiled wavefront correction, and low phototoxicity with a compact system. By tomographic imaging of the entire volume simultaneously, we obtained volumetric imaging across 225 × 225 × 16 μm3, with a resolution of up to 220 nm laterally and 400 nm axially, at the millisecond scale, over hundreds of thousands of time points. To establish the capabilities, we investigated large-scale cell migration and neural activities in different species and observed various subcellular dynamics in mammals during neutrophil migration and tumor cell circulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping