PUBLICATION
Abnormal brain development of monoamine oxidase mutant zebrafish and impaired social interaction of heterozygous fish
- Authors
- Baronio, D., Chen, Y.C., Panula, P.
- ID
- ZDB-PUB-211214-12
- Date
- 2021
- Source
- Disease models & mechanisms 15(3): (Journal)
- Registered Authors
- Baronio, Diego, Chen, Yu-Chia, Panula, Pertti
- Keywords
- Autism, Dopamine, Histamine, Parkinson's disease, Serotonin
- MeSH Terms
-
- Adult
- Animals
- Brain*/metabolism
- Humans
- Monoamine Oxidase*/genetics
- Monoamine Oxidase*/metabolism
- Serotonin/metabolism
- Social Interaction*
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 34881779 Full text @ Dis. Model. Mech.
Citation
Baronio, D., Chen, Y.C., Panula, P. (2021) Abnormal brain development of monoamine oxidase mutant zebrafish and impaired social interaction of heterozygous fish. Disease models & mechanisms. 15(3):.
Abstract
Monoamine oxidase (MAO) deficiency and imbalanced levels of brain monoamines have been associated with developmental delay, neuropsychiatric disorders and aggressive behavior. Animal models are valuable tools to gain mechanistic insight into outcomes associated with MAO deficiency. Here we report a novel genetic model to study the effects of mao-loss-of-function in zebrafish. Quantitative PCR, in situ hybridization and immunocytochemistry were used to study neurotransmitter systems, and expression of relevant genes for brain development in zebrafish mao mutants. Larval and adult fish behavior was evaluated through different tests. A stronger serotonin immunoreactivity was detected in both mao+/- and mao-/- larvae when compared with mao+/+ siblings. Mao-/- larvae were hypoactive, presented decreased reactions to visual and acoustic stimuli. They also had impaired histaminergic and dopaminergic systems, abnormal expression of developmental markers, and they died within 20 days post-fertilization. Mao+/- fish were viable, grew until adulthood and demonstrated anxiety-like behavior and impaired social interactions when compared with adult mao+/+ siblings. Our results indicate that mao-/- and mao+/- mutants could be promising tools to study the roles of MAO in brain development and behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping