PUBLICATION
Tissue distribution study of perfluorooctanoic acid in exposed zebrafish using MALDI mass spectrometry imaging
- Authors
- Bian, Y., He, M.Y., Ling, Y., Wang, X.J., Zhang, F., Feng, X.S., Zhang, Y., Xing, S.G., Li, J., Qiu, X., Li, Y.R.
- ID
- ZDB-PUB-211118-4
- Date
- 2021
- Source
- Environmental pollution (Barking, Essex : 1987) 293: 118505 (Journal)
- Registered Authors
- Li, Jie
- Keywords
- Environmental exposure, MALDI-MSI, Model fitting, PFOA, Zebrafish
- MeSH Terms
-
- Animals
- Caprylates*/pharmacokinetics
- Environmental Pollutants/pharmacokinetics*
- Fluorocarbons*/pharmacokinetics
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Tissue Distribution
- Zebrafish*
- PubMed
- 34785291 Full text @ Environ. Pollut.
Citation
Bian, Y., He, M.Y., Ling, Y., Wang, X.J., Zhang, F., Feng, X.S., Zhang, Y., Xing, S.G., Li, J., Qiu, X., Li, Y.R. (2021) Tissue distribution study of perfluorooctanoic acid in exposed zebrafish using MALDI mass spectrometry imaging. Environmental pollution (Barking, Essex : 1987). 293:118505.
Abstract
Perfluorooctanoic acid (PFOA) as an emerging environmental contaminant, has become ubiquitous in the environment. It is of significance to study bioconcentration and tissue distribution of aquatic organisms for predicting the persistence of PFOA and its adverse effects on the environment and human body. However, the distribution of PFOA in different tissues is a complex physiological process affected by many factors. It is difficult to be accurately described by a simple kinetic model. In present study, a new strategy was introduced to research the PFOA distribution in tissues and estimate the exposure stages. Zebrafish were continuously exposed to 25 mg/L PFOA for 30 days to simulate environmental process. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) method was used to monitor the spatio-temporal distribution of PFOA in zebrafish tissues. By analyzing the law of change obtained from the high spatial resolution MSI data, two different enrichment trends in ten tissues were summarized by performing curve fitting. Analyzing the ratio of two types of curves, a new "exposure curve" was defined to evaluate the exposure stages. With this model, three levels (mild, moderate, and deep pollution stage) of PFOA pollution in zebrafish can be simply evaluated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping