PUBLICATION
Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?
- Authors
- Félix, R.C., Anjos, L., Costa, R.A., Letsiou, S., Power, D.M.
- ID
- ZDB-PUB-211025-19
- Date
- 2021
- Source
- Marine drugs 19(10): (Journal)
- Registered Authors
- Keywords
- electric cell impedance sensing (ECIS), fish skin fibroblast, scratch assay, vertebrate CRTAC1, zebrafish
- MeSH Terms
-
- Animals
- Aquatic Organisms
- Calcium-Binding Proteins/chemistry
- Calcium-Binding Proteins/pharmacology*
- Calcium-Binding Proteins/therapeutic use
- Fibroblasts/drug effects*
- Humans
- Models, Animal
- Wound Healing/drug effects
- Zebrafish*
- PubMed
- 34677440 Full text @ Mar. Drugs
Citation
Félix, R.C., Anjos, L., Costa, R.A., Letsiou, S., Power, D.M. (2021) Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?. Marine drugs. 19(10):.
Abstract
Fish skin has been gaining attention due to its efficacy as a human-wound-treatment product and to identify factors promoting its enhanced action. Skin fibroblasts have a central role in maintaining skin integrity and secrete extra cellular matrix (ECM) proteins, growth factors and cytokines to rapidly repair lesions and prevent further damage or infection. The effects on scratch repair of the ubiquitous but poorly characterized ECM protein, cartilage acidic protein 1 (CRTAC1), from piscine and human sources were compared using a zebrafish SJD.1 primary fibroblast cell line. A classic in vitro cell scratch assay, immunofluorescence, biosensor and gene expression analysis were used. Our results demonstrated that the duplicate sea bass Crtac1a and Crtac1b proteins and human CRTAC-1A all promoted SJD.1 primary fibroblast migration in a classic scratch assay and in an electric cell impedance sensing assay. The immunofluorescence analysis revealed that CRTAC1 enhanced cell migration was most likely caused by actin-driven cytoskeletal changes and the cellular transcriptional response was most affected in the early stage (6 h) of scratch repair. In summary, our results suggest that CRTAC1 may be an important factor in fish skin promoting damage repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping