PUBLICATION
Alteration of organ size and allometric scaling by organ-specific targeting of IGF signaling
- Authors
- Kamei, H., Duan, C.
- ID
- ZDB-PUB-211005-17
- Date
- 2021
- Source
- General and comparative endocrinology 314: 113922 (Journal)
- Registered Authors
- Duan, Cunming
- Keywords
- Akt, Eye, Heart, Inner ear, Insulin-like growth factor 1 receptor, Insulin-like growth factor binding protein, Organ growth, Zebrafish
- MeSH Terms
-
- Animals
- Ear, Inner/growth & development
- Eye/growth & development
- Heart/growth & development
- Insulin-Like Growth Factor Binding Proteins*/genetics
- Insulin-Like Growth Factor Binding Proteins*/metabolism
- Insulin-Like Growth Factor I*/genetics
- Insulin-Like Growth Factor I*/metabolism
- Organ Size
- Phosphorylation
- Signal Transduction
- Somatomedins*/metabolism
- Zebrafish*/growth & development
- Zebrafish*/metabolism
- Zebrafish Proteins*/metabolism
- PubMed
- 34606746 Full text @ Gen. Comp. Endocrinol.
Citation
Kamei, H., Duan, C. (2021) Alteration of organ size and allometric scaling by organ-specific targeting of IGF signaling. General and comparative endocrinology. 314:113922.
Abstract
The size of an organ is proportional to the other body parts or the whole body. This relationship is known as allometry. Understanding how allometry is determined is a fundamental question in biology. Here we tested the hypothesis that local insulin-like growth factor (Igf) signaling is critical in regulating organ size and its allometric scaling by organ-specific expression of Igf binding proteins (Igfbp). Overexpression of Igfbp2a or 5b in the developing zebrafish eye, heart, and inner ear resulted in a disproportional reduction in their growth relative to the body. Stable transgenic zebrafish with lens-specific Igfbp5b expression selectively reduced adult eye size. The action is Igf-dependent because an Igf-binding deficient Igfbp5b mutant had no effect. Targeted expression of a dominant-negative Igf1 receptor (dnIgf1r) in the lens caused a similar reduction in relative eye growth. Furthermore, co-expression of IGF-1 with an Igfbp restored the eye size. Finally, co-expression of a constitutively active form of Akt with Igfbp or dnIgf1r restored the relative eye growth. These data suggest that local Igf availability and Igf signaling activity are critical determinants of organ size and allometric scaling in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping