PUBLICATION
Supplementation of zebrafish (Danio rerio) diet using a short antimicrobial peptide: Evaluation of growth performance, immunomodulatory function, antioxidant activity, and disease resistance
- Authors
- Rashidian, G., Moghaddam, M.M., Mirnejad, R., Azad, Z.M.
- ID
- ZDB-PUB-211002-12
- Date
- 2021
- Source
- Fish & shellfish immunology 119: 42-50 (Journal)
- Registered Authors
- Keywords
- Antimicrobial peptide, Immune and antioxidant genes, Streptococcus iniae, Yersinia ruckeri, Zebrafish
- MeSH Terms
-
- Animal Feed/analysis
- Animals
- Anti-Bacterial Agents/pharmacology
- Antimicrobial Cationic Peptides
- Antimicrobial Peptides
- Antioxidants
- Diet/veterinary
- Dietary Supplements
- Disease Resistance
- Fish Diseases*
- Streptococcal Infections*/veterinary
- Streptococcus iniae
- Yersinia Infections*/veterinary
- Zebrafish
- PubMed
- 34597813 Full text @ Fish Shellfish Immunol.
Citation
Rashidian, G., Moghaddam, M.M., Mirnejad, R., Azad, Z.M. (2021) Supplementation of zebrafish (Danio rerio) diet using a short antimicrobial peptide: Evaluation of growth performance, immunomodulatory function, antioxidant activity, and disease resistance. Fish & shellfish immunology. 119:42-50.
Abstract
Short-chain bioactive peptides are new and promising antimicrobial, immune moderating, and antioxidant agents. Therefore, the present study was conducted to evaluate in vitro antibacterial activity of CM11, a short antimicrobial peptide (AMP), against Streptococcus iniae and Yersinia ruckeri as fish pathogenic bacteria using standard disk diffusion and microdilution assays. In addition, in vivo effects of CM11 on fish growth, immunity, antioxidant activity, and disease resistance were evaluated using zebrafish (Danio rerio) as an animal model. For in vivo study, based on susceptibility results in vitro, four diets were designed to include zero (as control), 10, 20, and 50 μg of CM11 per g diet referred to as control, P1, P2, and P3 treatments, respectively. After eight weeks of dietary trial, fish were challenged with Streptococcus iniae, and the survival rate was calculated for a period of two weeks. Results showed that CM11 effectively inhibited the growth of S. iniae and Y. ruckeri on agar plates at a concentration of eight μg/ml. Minimum inhibitory and minimum bactericidal concentrations of CM11 were measured at 8 and 32 μg/ml for S. iniae and 16 and 64 μg/ml Y. ruckeri, respectively. In vivo results showed no noticeable effects on fish growth parameters, however, feed conversion ratio (FCR) was found lower in P3 and P2 compared to control (P < 0.05). Immunological and antioxidant responses were found strongly affected by CM11 in all treatment groups in which the highest values were found in the P3 treated group. Key immune and antioxidant genes were up-regulated particularly in fish receiving the highest level of CM11 (P3). Fish receiving the CM11 peptide showed better survival when challenged with S. iniae. These findings suggest the potential of CM11 for use in aquaculture as an antibacterial and immunostimulant agent.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping