PUBLICATION
IL-6 Signaling Protects Zebrafish Larvae during Staphylococcus epidermidis Infection in a Bath Immersion Model
- Authors
- Dhanagovind, P.T., Kujur, P.K., Swain, R.K., Banerjee, S.
- ID
- ZDB-PUB-210922-23
- Date
- 2021
- Source
- Journal of immunology (Baltimore, Md. : 1950) 207(8): 2129-2142 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cells, Cultured
- Cross Infection
- Disease Models, Animal
- Disease Resistance
- Humans
- Interleukin-6/metabolism*
- Larva
- MicroRNAs/genetics
- NF-kappa B/genetics
- NF-kappa B/metabolism*
- Signal Transduction
- Staphylococcal Infections/immunology*
- Staphylococcus epidermidis/physiology*
- Toll-Like Receptor 2/metabolism
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 34544800 Full text @ J. Immunol.
Citation
Dhanagovind, P.T., Kujur, P.K., Swain, R.K., Banerjee, S. (2021) IL-6 Signaling Protects Zebrafish Larvae during Staphylococcus epidermidis Infection in a Bath Immersion Model. Journal of immunology (Baltimore, Md. : 1950). 207(8):2129-2142.
Abstract
The host immune responses to Staphylococcus epidermidis, a frequent cause of nosocomial infections, are not well understood. We have established a bath immersion model of this infection in zebrafish (Danio rerio) larvae. Macrophages play a primary role in the host immune response and are involved in clearance of infection in the larvae. S. epidermidis infection results in upregulation of tlr-2 There is marked inflammation characterized by heightened NF-κB signaling and elevation of several proinflammatory cytokines. There is rapid upregulation of il-1b and tnf-a transcripts, whereas an increase in il-6 levels is relatively more delayed. The IL-6 signaling pathway is further amplified by elevation of IL-6 signal transducer (il-6st) levels, which negatively correlates with miRNA dre-miR-142a-5p. Enhanced IL-6 signaling is protective to the host in this model as inhibition of the signaling pathway resulted in increased mortality upon S. epidermidis infection. Our study describes the host immune responses to S. epidermidis infection, establishes the importance of IL-6 signaling, and identifies a potential role of miR-142-5p-il-6st interaction in this infection model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping