PUBLICATION
Nesfatin-1-Like Peptide is a Negative Regulator of Cardiovascular Functions in Zebrafish and Goldfish
- Authors
- Sundarrajan, L., Jayakumar Rajeswari, J., Weber, L.P., Unniappan, S.
- ID
- ZDB-PUB-210829-10
- Date
- 2021
- Source
- General and comparative endocrinology 313: 113892 (Journal)
- Registered Authors
- Unniappan, Suraj, Weber, Lynn
- Keywords
- Cardiovascular physiology, Goldfish, Heart, Nesfatin-1, Nesfatin-1-Like Peptide, Zebrafish
- MeSH Terms
-
- Animals
- Calcium-Binding Proteins/genetics
- Calcium-Binding Proteins/metabolism
- Cardiovascular Physiological Phenomena
- Goldfish*/genetics
- Peptides/metabolism
- Rats
- Receptors for Activated C Kinase
- Zebrafish*/metabolism
- PubMed
- 34453930 Full text @ Gen. Comp. Endocrinol.
Citation
Sundarrajan, L., Jayakumar Rajeswari, J., Weber, L.P., Unniappan, S. (2021) Nesfatin-1-Like Peptide is a Negative Regulator of Cardiovascular Functions in Zebrafish and Goldfish. General and comparative endocrinology. 313:113892.
Abstract
Nucleobindins (NUCB1 and NUCB2) were originally identified as calcium and DNA binding proteins. Nesfatin-1 (NEFA/nucleobindin-2-Encoded Satiety and Fat-Influencing proteiN-1) is an 82 amino acid anorexigenic peptide encoded in the N-terminal region of NUCB2. We have shown that nesfatin-1 is a cardio suppressor in zebrafish. Both NUCB1 and NUCB2 possess a nesfatin-1-like peptide with a very highly conserved bioactive core. It was found that a nesfatin-1-like peptide (NLP) encoded in NUCB1 suppresses food intake in fish. In this research, we investigated whether NLP has nesfatin-1-like effects on cardiovascular functions. NUCB1/NLP-like immunoreactivity was found in the atrium and ventricle of the heart and skeletal muscle of zebrafish. Intraperitoneal injection (IP) of either zebrafish NLP or rat NLP suppressed cardiac functions in both zebrafish and goldfish. Irisin and RyR1b mRNA expression was downregulated by NLP in zebrafish cardiac and skeletal muscles. However, cardiac ATP2a2 mRNA expression was elevated by NLP injection. Administration of scrambled NLP did not affect irisin, RyR1b or ATP2a2 mRNA expression in zebrafish. Together, these novel results implicate NLP as a suppressor of cardiovascular physiology in zebrafish and goldfish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping