PUBLICATION
Fate restriction and multipotency in retinal stem cells
- Authors
- Centanin, L., Hoeckendorf, B., Wittbrodt, J.
- ID
- ZDB-PUB-210827-21
- Date
- 2011
- Source
- Cell Stem Cell 9: 553-62 (Journal)
- Registered Authors
- Centanin, Lazaro, Wittbrodt, Jochen
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Differentiation/physiology*
- Cell Lineage
- Cells, Cultured
- Multipotent Stem Cells/cytology*
- Multipotent Stem Cells/physiology*
- Oryzias/anatomy & histology
- Oryzias/embryology
- Oryzias/growth & development
- Retina/cytology*
- Retina/embryology
- Retina/growth & development
- Retinal Pigment Epithelium/cytology
- PubMed
- 22136930 Full text @ Cell Stem Cell
Citation
Centanin, L., Hoeckendorf, B., Wittbrodt, J. (2011) Fate restriction and multipotency in retinal stem cells. Cell Stem Cell. 9:553-62.
Abstract
Stem cells have the capacity to both self-renew and generate postmitotic cells. Long-term tracking of individual clones in their natural environment constitutes the ultimate way to validate postembryonic stem cells. We identify retinal stem cells (RSCs) using the spatiotemporal organization of the fish retina and follow the complete offspring of a single cell during the postnatal life. RSCs generate two tissues of the adult fish retina, the neural retina (NR) and the retinal-pigmented epithelium (RPE). Despite their common embryonic origin and tight coordination during continuous organ growth, we prove that NR and RPE are maintained by dedicated RSCs that contribute in a fate-restricted manner to either one or the other tissue. We show that in the NR, RSCs are multipotent and generate all neuron types and glia. The clonal origin of these different cell types from a multipotent NSC has far-reaching implications for cell type and tissue homeostasis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping