PUBLICATION
Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
- Authors
- Lepanto, P., Levin-Ferreyra, F., Koziol, U., Malacrida, L., Badano, J.L.
- ID
- ZDB-PUB-210820-3
- Date
- 2021
- Source
- Biology Open 10(9): (Journal)
- Registered Authors
- Badano, Jose, Lepanto, Paola
- Keywords
- Adipocyte, Blood vessels, Nile Red, Zebrafish
- MeSH Terms
-
- Adipocytes/physiology*
- Adipogenesis/genetics*
- Adiponectin/metabolism
- Adipose Tissue, White/cytology*
- Animals
- Animals, Genetically Modified
- CCAAT-Enhancer-Binding Proteins/metabolism
- Cell Differentiation/genetics*
- Cell Line
- Complement Factor D/metabolism
- Endothelial Cells/physiology
- Fatty Acid-Binding Proteins/metabolism
- Zebrafish/embryology*
- PubMed
- 34409430 Full text @ Biol. Open
Citation
Lepanto, P., Levin-Ferreyra, F., Koziol, U., Malacrida, L., Badano, J.L. (2021) Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish. Biology Open. 10(9):.
Abstract
White adipose tissue hyperplasia has been shown to be crucial for handling excess energy in healthy ways. Though adipogenesis mechanisms have been underscored in vitro, we lack information on how tissue and systemic factors influence the differentiation of new adipocytes. While this could be studied in zebrafish, adipocyte identification currently relies on neutral lipid labeling, thus precluding access to cells in early stages of differentiation. Here we report the generation and analysis of a zebrafish line with the transgene fabp4a(-2.7):EGFPcaax. In vivo confocal microscopy of the pancreatic and abdominal visceral depots of transgenic larvae, revealed the presence of labeled mature adipocytes as well as immature cells in earlier stages of differentiation. Through co-labeling for blood vessels, we observed a close interaction of differentiating adipocytes with endothelial cells through cell protrusions. Finally, we implemented hyperspectral imaging and spectral phasor analysis in Nile Red labeled transgenic larvae and revealed the lipid metabolic transition towards neutral lipid accumulation of differentiating adipocytes. Altogether our work presents the characterization of a novel adipocyte-specific label in zebrafish and uncovers previously unknown aspects of in vivo adipogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping