PUBLICATION
A Simple Method to Establish Metaphase Chromosomes from Individual Zebrafish Embryos
- Authors
- Asana Marican, H.T., Sun, L.W.H., Shen, H.
- ID
- ZDB-PUB-210813-7
- Date
- 2021
- Source
- Zebrafish 18(5): 338-341 (Journal)
- Registered Authors
- Shen, Hongyuan
- Keywords
- cytogenetics, genomic instability, metaphase chromosomes, zebrafish
- MeSH Terms
-
- Animals
- Chromosome Aberrations
- Chromosomes*
- Cytogenetics
- Metaphase
- Zebrafish*/genetics
- PubMed
- 34382863 Full text @ Zebrafish
Citation
Asana Marican, H.T., Sun, L.W.H., Shen, H. (2021) A Simple Method to Establish Metaphase Chromosomes from Individual Zebrafish Embryos. Zebrafish. 18(5):338-341.
Abstract
Cytogenetic approach based on metaphase chromosomes established from dividing cells enables direct microscopic visualization of individual chromosomes, a powerful technique to investigate aneuploidy, chromosome aberrations, and genomic instability. In this study, we describe a simple method based on direct blocking of metaphases in individual zebrafish embryo and dropping slides with temperature changes, water vapor, and acetic acid treatment to increase the metaphase diameters. We demonstrate that well-separated metaphases could be established from single zebrafish embryos using this method. Our method could be further adapted for the analyses of DNA damage, chromosome aberrations, and genomic instability using zebrafish and other teleost models.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping