PUBLICATION
The edible Lactobacillus paracasei X11 with Konjac glucomannan promotes intestinal motility in zebrafish
- Authors
- Lu, Y., Zhang, J., Zhou, X., Guan, M., Zhang, Z., Liang, X., Tong, L., Yi, H., Gong, P., Bai, L., Zhou, H., Liu, T., Zhang, L.
- ID
- ZDB-PUB-210803-7
- Date
- 2021
- Source
- Neurogastroenterology and motility 33(12): e14196 (Journal)
- Registered Authors
- Keywords
- 5-HT, food ingredients, intestinal motility obstruction, peristalsis, probiotics, zebrafish
- MeSH Terms
-
- Animals
- Constipation/drug therapy
- Constipation/physiopathology
- Disease Models, Animal
- Gastrointestinal Motility/drug effects*
- Gastrointestinal Motility/physiology
- Intestinal Obstruction/drug therapy*
- Intestinal Obstruction/physiopathology
- Intestines/drug effects*
- Intestines/metabolism
- Lacticaseibacillus paracasei*
- Mannans/pharmacology*
- Mannans/therapeutic use
- Probiotics/pharmacology*
- Probiotics/therapeutic use
- Serotonin/metabolism
- Zebrafish
- PubMed
- 34337833 Full text @ Neurogastroenterol. Motil.
Citation
Lu, Y., Zhang, J., Zhou, X., Guan, M., Zhang, Z., Liang, X., Tong, L., Yi, H., Gong, P., Bai, L., Zhou, H., Liu, T., Zhang, L. (2021) The edible Lactobacillus paracasei X11 with Konjac glucomannan promotes intestinal motility in zebrafish. Neurogastroenterology and motility. 33(12):e14196.
Abstract
Background Constipation is a gastrointestinal symptom with high incidence rate and large number of patients. It is becoming one of the urgent medical problems. Poor intestinal motility is one of the important causes of constipation. Current drug treatments for constipation are associated with many side effects; thus, it is necessary to study more effective treatment methods and potential mechanism.
Methods A zebrafish model of intestinal motility obstruction was established by loperamide hydrochloride to evaluate the effect of probiotic, food ingredients, and combination on intestinal peristalsis according to intestinal peristalsis frequency counts. The gastrointestinal survival ability of the best probiotics was evaluated by surface hydrophobicity, self-aggregation, acid and bile salt tolerance, and gastrointestinal transit tolerance. Interactions between probiotics and food ingredients were studied in vivo and in vitro. The expression of 5-HT was detected by ELISA and fluorescence immunoassay, and 5-HT related genes were detected by RT-PCR.
Key results We obtained the probiotics, food ingredients, and combination that effectively promoted intestinal peristalsis, X11 and YRL577, P. persica and KGM, KGM + X11, respectively. Both KGM and P. persica promoted colonization of probiotics in vivo. KGM + X11 could effectively promote the increase in 5-HT synthesis in zebrafish via up-regulating gene expression of TPH-1, TPH-2, and 5-HTR and down-regulating gene expression of SERT. The specific in-depth mechanism needs further study.
Conclusions and inferences The combinations of KGM with X11 effectively promoted intestinal peristalsis. We provide a theoretical basis for new modalities that can promote intestinal peristalsis and alleviate constipation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping