PUBLICATION
Zebrafish gasdermin E cleavage-engaged pyroptosis by inflammatory and apoptotic caspases
- Authors
- Chen, H., Wu, X., Gu, Z., Chen, S., Zhou, X., Zhang, Y., Liu, Q., Wang, Z., Yang, D.
- ID
- ZDB-PUB-210714-21
- Date
- 2021
- Source
- Developmental and comparative immunology 124: 104203 (Journal)
- Registered Authors
- Yang, Dahai
- Keywords
- Apoptotic caspases, Gsdme, Inflammatory caspases, Pyroptosis, Zebrafish
- MeSH Terms
-
- Amino Acid Motifs
- Animals
- Anti-Bacterial Agents/metabolism
- Caspases/metabolism*
- Escherichia coli/growth & development
- Escherichia coli/metabolism
- HEK293 Cells
- Humans
- Pore Forming Cytotoxic Proteins/chemistry
- Pore Forming Cytotoxic Proteins/metabolism*
- Pyroptosis*
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 34252476 Full text @ Dev. Comp. Immunol.
Citation
Chen, H., Wu, X., Gu, Z., Chen, S., Zhou, X., Zhang, Y., Liu, Q., Wang, Z., Yang, D. (2021) Zebrafish gasdermin E cleavage-engaged pyroptosis by inflammatory and apoptotic caspases. Developmental and comparative immunology. 124:104203.
Abstract
As the executor of pyroptosis known to date, gasdermins (GSDMs), consists of GSDMA, GSDMB, GSDMC, GSDMD, GSDME and pejvakin, might play critical roles in anti-bacterial infection as well as inflammatory diseases. However, zebrafish only harbors a pair of Gsdme (Gsdmea/b), and their activation mechanisms remain largely unknown. Herein, we investigate the activation mechanism of Gsdmea/b cleaved by inflammatory and apoptotic caspases in zebrafish，and found that Gsdmea/b are equally cleaved by Caspase 19b, a sister of Caspy2, but not Caspy. Moreover, the zebrafish apoptotic effector caspases, including Caspase 3a/b and Caspase 7, also can cleave Gsdmea/b at the same sites as inflammatory caspases recognized. Importantly, our results reveal that Caspase 8a/b can cleave Gsdmeb, but only Caspase 8a can cleave Gsdmea. Taken together, these findings suggest that zebrafish Gsdmea/b can concurrently function as GSDMD and GSDME in mammals, which will contribute to better understanding the mechanism of pyroptosis activation in teleost, as well as provide a clue for drug screening model against inflammatory diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping