PUBLICATION
Characterization of SR-B2a and SR-B2b genes and their ability to promote GCRV infection in grass carp (Ctenopharyngodon idellus)
- Authors
- Li, Y., Huang, R., Chen, L., Li, Y., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y.
- ID
- ZDB-PUB-210713-2
- Date
- 2021
- Source
- Developmental and comparative immunology 124: 104202 (Journal)
- Registered Authors
- Zhu, Zuoyan
- Keywords
- GCRV, Grass carp, Innate immunity, Scavenger receptor class B type 2
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carps/genetics
- Carps/immunology
- Carps/virology*
- Cell Line
- Cell Membrane/metabolism
- Cytoplasm/metabolism
- Fish Diseases/genetics
- Fish Diseases/immunology
- Fish Diseases/virology
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Gene Expression
- Immunity, Innate
- Phylogeny
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reoviridae/pathogenicity*
- Reoviridae Infections/genetics
- Reoviridae Infections/immunology
- Reoviridae Infections/veterinary
- Reoviridae Infections/virology
- Scavenger Receptors, Class B/genetics
- Scavenger Receptors, Class B/physiology*
- Sequence Alignment
- Tissue Distribution
- Viral Load/genetics
- PubMed
- 34246624 Full text @ Dev. Comp. Immunol.
Citation
Li, Y., Huang, R., Chen, L., Li, Y., Li, Y., Liao, L., He, L., Zhu, Z., Wang, Y. (2021) Characterization of SR-B2a and SR-B2b genes and their ability to promote GCRV infection in grass carp (Ctenopharyngodon idellus). Developmental and comparative immunology. 124:104202.
Abstract
Scavenger receptor class B type 2 (SR-B2) is a pattern recognition receptor involved in innate immunity in mammals; however, the immunological function of SR-Bs in fish remains unclear. In this study, the full-length cDNA sequences of SR-B2a and SR-B2b from grass carp (Ctenopharyngodon idellus) were cloned and designated as CiSR-B2a and CiSR-B2b. Multiple alignments and phylogenetic analyses deduced that CiSR-B2a and CiSR-B2b had the highest evolutionary conservation and were closely related to the zebrafish (Danio rerio) homologues, DrSR-B2a and DrSR-B2b, respectively. Both CiSR-B2a and CiSR-B2b were expressed in all the tested tissues, with the highest expression levels found in the hepatopancreas. In Ctenopharyngodon idellus kidney cells (CIK), CiSR-B2a and CiSR-B2b were mainly located in the cytoplasm, and a small amount located on the plasma membrane. After challenge with Grass Carp Reovirus (GCRV), the expression of CiSR-B2a and CiSR-B2b were significantly upregulated in the spleen (about 10.27 and 27.19 times higher than that at 0 day, p < 0.01). With CiSR-B2a or CiSR-B2b overexpressed in CIK, the relative copy number of GCRV in the cells was both significantly increased compared to that in the control group, indicating that CiSR-B2a and CiSR-B2b may be important proteins during the infection processes of GCRV.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping