PUBLICATION
Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2
- Authors
- Balaraju, A.K., Hu, B., Rodriguez, J.J., Murry, M., Lin, F.
- ID
- ZDB-PUB-210705-4
- Date
- 2021
- Source
- Development (Cambridge, England) 148(14): (Journal)
- Registered Authors
- Lin, Fang
- Keywords
- Cadherin 2, Endoderm C&E, Glypican 4, Planar cell polarity, Zebrafish
- MeSH Terms
-
- Animals
- Cadherins/metabolism*
- Cell Polarity/physiology*
- Endoderm/metabolism*
- Gastrulation
- Gene Expression Regulation, Developmental
- Glypicans/metabolism*
- Heparan Sulfate Proteoglycans/metabolism
- Morphogenesis
- Wnt Proteins/metabolism
- Wnt Signaling Pathway
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism*
- rab5 GTP-Binding Proteins
- PubMed
- 34131730 Full text @ Development
Citation
Balaraju, A.K., Hu, B., Rodriguez, J.J., Murry, M., Lin, F. (2021) Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2. Development (Cambridge, England). 148(14):.
Abstract
Non-canonical Wnt/Planar Cell Polarity (Wnt/PCP) signaling has been implicated in endoderm morphogenesis. However, the underlying cellular and molecular mechanisms of this process are unclear. We found that during convergence and extension (C&E) in zebrafish, gut endodermal cells are polarized mediolaterally, with GFP-Vangl2 enriched at the anterior edges. Endoderm cell polarity is lost, and intercalation is impaired, in the absence of glypican 4 (gpc4), a heparan-sulfate proteoglycan that promotes Wnt/PCP signaling, suggesting that this signaling is required for endodermal cell polarity. Live imaging revealed that endoderm C&E is accomplished by polarized cell protrusions and junction remodeling, which are impaired in gpc4-deficient endodermal cells. Furthermore, in the absence of gpc4, Cadherin 2 expression on the endodermal cell surface is increased due to impaired Rab5c-mediated endocytosis, which partially accounts for the endodermal defects in these mutants.These findings indicate that Gpc4 regulates endodermal planar cell polarity during endoderm C&E by influencing localization of Cadherin 2. Thus, our study uncovers a new mechanism by which Gpc4 regulates planar cell polarity and reveals the role of Wnt/PCP signaling in endoderm morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping